METHANOL
CASRN: 67-56-1

See Occupational Exposure Standards

Human Health Effects:

Toxicity Summary:

Methanol occurs naturally in humans, animals and plants. It is a natural constituent in blood, urine, saliva and expired air. ... The two most important sources of background body burdens for methanol and formate are diet and metabolic processes. Methanol is available in the diet principally from fresh fruits and vegetables, fruit juices ... fermented beverages ... and diet foods (principally soft drinks). The artificial sweetner aspartame is widely used and, on hydrolysis, 10% (by weight) of the molecule is converted to free methanol, which is available for absorption. ... Exposures to methanol can occur in occupational settings through inhalation or dermal contact. ... Methanol is readily absorbed by inhalation, ingestion and dermal exposure, and it is rapidly distributed to tissues according to the distribution of body water. A small amount of methanol is excreted unchanged by the lungs and kidneys. ... Methanol is metabolized primarily in the liver by sequential oxidative steps to formaldehyde, formic acid and carbon dioxide. The initial step involves oxidation to formaldehyde by hepatic alcohol dehydrogenase ... In step 2, formaldehyde is oxidized by formaldehyde dehydrogenase to formic acid/or formate depending on the pH. In step 3, formic acid is detoxified to carbon dioxide by folate-dependent reactions. Elimination of methanol from the blood via the urine and exhaled air and by metabolism appears to be slow in all species, especially when compared to ethanol. ... It is the rate of metabolic detoxification, or removal of formate that is vastly different between rodents and primates and is the basis for the dramatic differences in methanol toxicity observed between rodents and primates. The acute and short term toxicity of methanol varies greatly between different species, toxicity being highest in species with a relatively poor ability to metabolize formate. In such cases of poor metabolism of formate, fatal methanol poisoning occurs as a result of metabolic acidosis and neuronal toxicity, whereas, in animals that readily metabolize formate, consequences of CNS depression (coma, respiratory failure, etc.) are usually the cause of death. Sensitive primate species (humans and monkeys) develop increased blood formate concentrations following methanol exposure, while resistant rodents, rabbits and dogs do not. Humans and non-human primates are uniquely sensitive to the toxic effects of methanol. Overall methanol has a low acute toxicity to non-primate animals. ... In the rabbit, methanol is a moderate irritant to the eye. It was not skin sensitizing ... There is no evidence from animal studies to suggest that methanol is a carcinogen ... The inhalation of methanol by pregnant rodents throughout the period of embryogenesis induces a wide range of concentration-dependent teratogenic and embryolethal effects. Treatment-related malformations, primarily extra or rudimentary cervical ribs and urinary or cardiovascular defects, were found in fetuses of rats ... Increased incidences of exencephaly and cleft palate were found in the offspring of ... mice ... There was increased embryo/fetal death ... and an increasing incidence of full litter resorptions. Reduced fetal weight was observed ... Fetal malformations ... included neural and ocular defects, cleft palate, hydronephrosis and limb anomalies. Humans (and non-human primates) are uniquely sensitive to methanol poisoning and the toxic effects in these species are characterized by formic acidemia, metabolic acidosis, ocular toxicity, nervous system depression, blindness, coma and death. Nearly all of the available information on methanol toxicity in humans relates to the consequences of acute rather than chronic exposures. A vast majority of poisonings involving methanol have occurred from drinking adulterated beverages and from methanol-containing products. Although ingestion dominates as the most frequent route of poisoning, inhalation of high concentrations of methanol vapor and percutaneous absorption of methanolic liquids are as effective as the oral route in producing acute toxic effects. The most noted health consequences of longer term exposure to lower levels of methanol is a broad range of ocular effects. ... The toxicity is manifest if formate generation continues at a rate that exceeds its rate of metabolism. ... The minimum lethal dose of methanol in the absence of medical treatment is between 0.3 and 1 g/kg. The minimum dose causing permanent visual defects is unknown. ... Wide interindividual variability of the toxic dose is a prominent feature in acute methanol poisoning. Two important determinants of human susceptibility to methanol toxicity appear to be (1) concurrent ingestion of ethanol, which slows the entrance of methanol into the metabolic pathway, and (2) hepatic folate status, which governs the rate of formate detoxification. The symptoms and signs of methanol poisoning, which may not appear until after an asymptomatic period ... include visual disturbances, nausea, abdominal and muscle pain, dizziness, weakness and disturbances of consciousness ranging from coma to clonic seizures. Visual disturbances ... range from mild photophobia and misty or blurred vision to markedly reduced visual acuity and complete blindness. In extreme cases death results. The principal clinical feature is severe metabolic acidosis of the anion-gap type. The acidosis is largely attributed to the formic acid produced when methanol is metabolized. ... Visual disturbances of several types (blurring, constriction of the visible field, changes in color perception, and temporary or permanent blindness) have been reported in workers ... No other adverse effects of methanol have been reported in humans except minor skin and eye irritation. ... Methanol is of low toxicity to aquatic organisms, and effects due to environmental exposure to methanol are unlikely to be observed, except in the case of a spill.
[Environmental Health Criteria 196: Methanol pp. 1-9 (1997) by the International Programme on Chemical Safety (IPCS) under the joint sponsorship of the United Nations Environment Programme, the International Labour Organisation and the World Health Organization.]**QC REVIEWED**

Human Toxicity Excerpts:

... CHRONIC POISONING FROM REPEATED EXPOSURE TO ... VAPOR WERE MANIFESTED BY CONJUNCTIVITIS, HEADACHE, GIDDINESS, INSOMNIA, GASTRIC DISTURBANCES, & FAILURE OF VISION. ... ONE FATAL CASE OF OCCUPATIONAL ... INTOXICATION BY INHALATION. ...
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 372]**PEER REVIEWED**

POISONING ... RESULTS FROM A COMBINATION OF THE FOLLOWING: 1) A MINOR FACTOR OF CNS DEPRESSION, SIMILAR TO THAT PRODUCED BY ETHYL ALCOHOL; 2) A MAJOR FACTOR OF ACIDOSIS DUE TO FORMATION OF FORMIC & OTHER ORG ACIDS ... SPECIFIC TOXICITY OF OXIDATION PRODUCTS ... (PROBABLY FORMALDEHYDE) FOR RETINAL CELLS. SYMPTOMS ... OF METHANOL POISONING CONSIST OF HEADACHE, VERTIGO, VOMITING, SEVERE UPPER ABDOMINAL PAIN, BACK PAIN, DYSPNEA, MOTOR RESTLESSNESS, COLD CLAMMY EXTREMITIES, BLURRING OF VISION, HYPEREMIA OF OPTIC DISC, ... DIARRHEA. ... PULSE IS SLOW IN SEVERELY ILL PT, & BRADYCARDIA CONSTITUTES GRAVE PROGNOSTIC SIGN.
[Gilman, A.G., L.S.Goodman, and A. Gilman. (eds.). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 7th ed. New York: Macmillan Publishing Co., Inc., 1985. 381]**PEER REVIEWED**

VISUAL DISTURBANCE CAN PROCEED TO BLINDNESS ... PUPILS THEN DO NOT REACT TO LIGHT. RESTLESSNESS & DELIRIUM ... COMA CAN DEVELOP WITH ... RAPIDITY ... RESP IS SLOW, SHALLOW, GASPING. ... DEATH MAY BE SUDDEN, OR ... AFTER MANY HR OF COMA. DEATH OCCURS IN INSPIRATORY APNEA, WITH TERMINAL OPISTHOTONUS & CONVULSIONS ... DEATH ... NEARLY ALWAYS PRECEDED BY BLINDNESS. AS LITTLE AS 4 ML OF METHANOL HAS CAUSED BLINDNESS, AND INGESTION OF 80-150 ML IS USUALLY FATAL. ... NEUROLOGICAL DAMAGE, GIVING RISE TO PERMANENT MOTOR DYSFUNCTION, MAY FOLLOW METHANOL POISONING.
[Gilman, A.G., L.S.Goodman, and A. Gilman. (eds.). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 7th ed. New York: Macmillan Publishing Co., Inc., 1985. 382]**PEER REVIEWED**

OPHTHALMOSCOPIC EXAM ... SHOWING HYPEREMIA ... OF OPTIC NERVEHEADS ... THEN ... EDEMA OF DISC MARGINS & ADJACENT RETINA. ... EDEMA ... APPEARING CHIEFLY IN NERVE FIBER LAYER & ... FOLLOW COURSE OF MAJOR RETINAL VESSELS. ... PERSISTING EDEMA OF RETINA ... WITH ATROPHY OF OPTIC NERVEHEAD ... & VISION COMPLETELY & PERMANENTLY GONE.
[Grant, W.M. Toxicology of the Eye. 3rd ed. Springfield, IL: Charles C. Thomas Publisher, 1986. 592]**PEER REVIEWED**

ACUTE METHANOL INTOXICATION IN 24 MEN: 9 HAD NO OCULAR EFFECTS; 7 HAD TRANSIENT EFFECTS: PERIPAPILLARY EDEMA, OPTIC DISC HYPEREMIA, DIMINISHED PUPILLARY LIGHT REACTION, CENTRAL SCOTOMA. EIGHT HAD PERMANENT OPTIC DISC PALLOR, ARTERIOLE ATTENUATION & SHEATHING, DIMINISHED PUPILLARY LIGHT REACTION, DIMINISHED VISUAL ACUITY, CENTRAL SCOTOMA, OTHER NERVE FIBER BUNDLE EFFECTS. COMPLETE BLINDNESS IN 2, SEVERE VISUAL DEFICIT IN 4.
[DETHLEFS R, NARAQI S; MED J AUST 2 (10): 483-5 (1978)]**PEER REVIEWED**

SYMPTOMATOLOGY: 1. A latency usually of 12-18 hours, during which time the only clinical signs are those of a generally mild and transient state of inebriation as after ethanol. 2. Headache, anorexia, weakness, fatigue, leg cramps, vertigo, restlessness. 3. Nausea, occasionally vomiting and diarrhea. Violent abdominal pain, back pain, leg pain. 4. Apathy or delirium progressing sometimes rapidly to coma. Rarely excitement, mania, and convulsions. 5. Dimness of vision with dilated pupils, reacting poorly, if at all, to light, followed often by bilateral blindness (transient or permanent). Eyes are often sensitive to pressure, and eye movements are painful. 6. Breathing is rapid and shallow, not usually deep and labored as seen in other types of metabolic acidosis. 7. Mild tachycardia is common, but the blood pressure is usually well maintained. 8. Death in coma is due to respiratory failure or rarely to circulatory collapse. 9. Protracted convalescence with asthenia. Blindness is usually permanent.
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.,p. III-277]**PEER REVIEWED**

The NIOSH review of the literature failed to reveal any epidemiologic surveys sufficiently comprehensive to bear significantly on the workplace environmental limit. A report ... indicated severe recurrent headaches in workers exposed to methyl alcohol in concentrations between 200-375 ppm. Diminution of vision was reported from airborne methyl alcohol concentrations of 1200 to 8300 ppm.
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 372]**PEER REVIEWED**

Two cases which were described as multiple neuritis in men engaged in shellacking furniture with shellac dissolved in methyl alcohol /were reported/. Symptoms reported were paresthesia, numbing, prickling, and shooting pain in the back of the hands and forearms, in addition to edema of the arms. Both men sought medical aid promptly, and the resultant cessation of exposure probably prevented the development of serious sequelae of methyl alcohol intoxication. It was considered that these 2 cases were due to the inhalation of the vapor of the wood alcohol employed.
[Jelliffe SE; Med News 86: 387-90 (1905) as cited in NIOSH; Criteria Document: Methyl Alcohol p.27-8 (1976) DHEW Pub. NIOSH 76-148]**PEER REVIEWED**

The case of a businessman who had been in the habit of drinking quite regularly, in small quantities, for a period of at least 3 months an illicit whiskey which apparently contained 35% Columbian spirits (methyl alcohol). When observed, the subject was suffering from severe gastric irritability, marked hyperesthesia in both arms and hands, incomplete paralysis of the extensors, and waist drop. He also had a mild degree of ptosis of the eyelids and a restricted partial amblyopia. He recovered after 4 months of treatment but still had some residual blurring of vision. In summary, researchers commented upon a postulated "greater susceptibility of the ganglion cells of the retina" to poisoning by methyl alcohol.
[Jelliffe SE; Med News 86: 387-90 (1905) as cited in NIOSH; Criteria Document: Methyl Alcohol p.28 (1976) DHEW Pub. NIOSH 76-148]**PEER REVIEWED**

Effects seen from either of the 2 most common routes of occupational exposure (inhalation and percutaneous absorption) include: headache, dizziness, nausea, vomiting, weakness, vertigo, chills, shooting pains in the lower extremities, unsteady gait, dermatitis, multiple neuritis characterized by paresthesia, numbness, prickling, and shooting pain in the back of the hands and forearms, as well as edema of the arms, nervousness, gastric pain, insomnia, acidosis, and formic acid in the urine. Eye effects, such as blurred vision, constricted visual fields, blindness, changes in color perception, double vision, and general visual disturbances have been reported. Eye examination have shown sluggish pupils, pallid optic discs, retinal edema, papilledema, hyperemia of the optic discs with blurred edges and dialated veins.
[NIOSH; Criteria Document: Methyl Alcohol p.68-9 (1976) DHEW Pub. NIOSH 76-148]**PEER REVIEWED**

A case of methyl alcohol poisoning in a worker who was involved in varnishing the inside of beer vats /is described/. Work was commenced on December 3, 1911, and continued on the following day with no medical complaints. On December 5, the worker experienced headache, vertigo, unsteady gait, nausea, vomiting, and acted as if intoxicated; consequently he did not work on this day. On December 7, the worker began having visual disturbances. At this time, he consulted a physician who diagnosed methyl alcohol poisoning. On December 12, an ophthalmologist made the following observations: the pupils were practically nonreactive to light, there was retinal edema, and initial vision (eccentric) was right 1/200 and left 2/200. In three weeks, his vision had improved to 20/30 in each eye. Six to 7 months later, with no additional methyl alcohol exposure, visual acuity remained stable, while pupillary response to light remained sluggish. In addition, researchers described a progressive contraction of the visual fields during the entire period of observation. ...The progressive constriction of visual fields corresponded to degenerated bundles of fibers and groups of ganglion cells becoming confluent as the degenerative process spread. It was concluded that this case was produced solely by inhalation of methyl alcohol vapor. The airborne concentration of methyl alcohol to which the worker was exposed was not determined.
[Tyson HH; Arch Ophthalmol 16: 459-71 (1912) as cited in NIOSH; Criteria Document: Methyl Alcohol p.29 (1976) DHEW Pub. NIOSH 76-148]**PEER REVIEWED**

At high concn methanol may cause optic atrophy and blindness, as well as dermatitis.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) PublicationNo. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981. 1]**PEER REVIEWED**

Skin, Eye and Respiratory Irritations:

/Methanol/ is a skin and eye irritant.
[Sax, N.I. Dangerous Properties of Industrial Materials. 6th ed. New York, NY: Van Nostrand Reinhold, 1984. 1765]**PEER REVIEWED**

Medical Surveillance:

The following medical procedures should be made available to each employee who is exposed to methyl alcohol at potentially hazardous levels: 1. A complete history and physical examination should be given to detect existing conditions that might place the employee at increased risk, and to establish a baseline for future health monitoring. Examination of the skin, liver, kidneys, and eyes should be stressed. Skin disease: Methyl alcohol is a defatting agent and can cause dermatitis on prolonged exposure. Persons with ... existing skin disorders may be susceptible to the effects of this agent. Liver function tests: Methyl alcohol may cause liver damage. A profile of liver function should be obtained by utilizing a medically acceptable array of biochemical tests. Kidney disease: Although methyl alcohol has not been proven to be kidney toxin in humans, the importance of this organ in the elimination of toxic substances justifies special consideration in those with impaired renal function. Eye disease: Because methyl alcohol may cause optic atrophy and blindness, those with existing eye diseases may be at increased risk from exposure. The aforementioned medical examinations should be repeated on an annual basis. In addition, anyone developing the above-listed conditions or who has been splashed in the eyes with, has ingested, or otherwise has been exposed to methyl alcohol should be placed under medical surveillance.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) PublicationNo. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981. 1]**PEER REVIEWED**

Special tests which may be used include: Determination of methyl alcohol in blood and methyl alcohol and formic acid in urine; estimation of alkali reserve which may be impaired because of acidosis following accidental ingestion.
[Sittig, M. Handbook of Toxic and Hazardous Chemicals and Carcinogens, 1985. 2nd ed. Park Ridge, NJ: Noyes Data Corporation, 1985. 583]**PEER REVIEWED**

A study was performed among 20 workers employed in a printing office at 3 different work places (methanol concentration of 85, 101 and 134 ppm) to determine whether the concentration of formic acid in blood or urine and the methanol content of alveolar air permit the estimation of methanol exposure. Blood, urine and end expiratory air were collected at the beginning and the end of the shift. For comparison formic acid concentrations were determined in the morning and in the afternoon in blood and urine of 36 and 15 control persons, respectively. The concentration of formic acid in blood increased significantly from 3.2:2.4 mg/l before to 7.9:3.2 mg/l after the shift in the exposed workers (mean increase 4.7:3.8 mg/l). The corresponding concentrations in urine were 13.1:5.3 mg/l. This difference is also significant. In the control groups there was a small but significant decrease of formic acid concentration in blood from 5.6:4.5 mg/l in the morning to 4.9:4.2 mg/l in the afternoon. In urine, the formic acid concentrations in the morning (11.9:6.4 mg/l) and in the afternoon (11.7:5.6 mg/l) were not significantly different. The increase of formic acid concentration in blood during the shift is the most useful parameter for monitoring methanol-exposed persons.
[Baumann K, Angerer J; Int Arch Occup Environ Health 42 (3-4): 241-9 (1979)]**PEER REVIEWED**

A sampling strategy was developed to detect personal exposure to methanol and formic acid vapors. Formic acid is the metabolic end product of methanol, and part of inhaled formic acid is excreted directly in urine, so that urinary formic acid would reveal exposure to both agents. A linear relationship to inhaled vapors, however, could be shown only if urinary sampling were delayed until 16 hr (next morning) after exposure. Exposure to methanol vapor at the current Finnish hygienic limit level (200 ppm) produced 80 mg formic acid/g creatinine; exposure to formic acid at the hygience limit (5 ppm) caused 90 mg/g creatinine. The similarity of these figures may indicate a common toxicological foundation of these empirically set values.
[Liesivuori J, Savolainen H; Am Ind Hyg Assoc J 48 (1): 32-34 (1987)]**PEER REVIEWED**

Headspace gas chromatography was used to determine the concentration of ethanol and methanol in blood samples from 519 individuals suspected of drinking and driving in Sweden where the legal alcohol limit is 0.50 mg/g in whole blood (11 mmol/l). The concentration of ethanol in blood ranged from 0.01 to 3.52 mg/g with a mean of 1.83 + or - 0.82 mg/g (+ or - standard deviation). The frequency distribution was symmetrical about the mean but deviated from normality. A plot of the same data on normal probability paper indicated that it might be composed of two subpopulations (bimodal). The concentration of methanol in the same blood specimens ranged from 1 to 23 mg/l with a mean of 7.3 + or - 3.6 mg/l (+ or - standard deviation) and this distribution was markedly skew (+). The concentration of ethanol (x) and methanol (y) were positively correlated (r= 0.47, P less than 0.001) and implies that 22% (r2) of the variance in blood-methanol can be attributed to its linear regression on blood-ethanol. The regression equation was y= 3.6 + 2.1 x and the standard error estimate was 0.32 mg/l. This large scatter precludes making reliable estimates of blood-methanol concentrations are definitely associated with higher blood-ethanol in ths sample of Swedish drinking drivers. Frequent exposure to methanol and its toxic products of metabolism, formaldehyde and formic acid, might constitute an additional health risk associated with heavy drinking in predisposed individuals. The determination of methanol in blood of drinking drivers in addition to ethanol could indicate long-standing ethanol intoxication and therfore potential problem drinkers or alcoholics.
[Jones AW, Lowinger H; Forensic Sci Int 37 (4): 277-85 (1988)]**PEER REVIEWED**

Populations at Special Risk:

Persons with existing skin, kidney, liver, or eye disorders may be at an increased risk when exposed to methanol.
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) PublicationNo. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981. 1]**PEER REVIEWED**

Probable Routes of Human Exposure:

... eye contact
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) PublicationNo. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981. 1]**PEER REVIEWED**

The general population is exposed to methanol through inhalation of air, through consumption of various drinking waters and foods, and through dermal contact of various consumer products such as paint thinners and strippers, adhesives, cleaners, and inks. Widespread occupational exposure occurs through inhalation and dermal contact. (SRC)
**PEER REVIEWED**

STUDY OF WOOD HEEL INDUSTRY IN MA SHOWED AVG METHANOL VAPOR CONCN RANGING FROM 160-170 PPM, WITH NO DEFINITE EVIDENCE OF INJURY TO EXPOSED WORKERS ... CONCN BETWEEN 400 & 1000 PPM IN SPIRIT DUPLICATING PROCESSES /WERE REPORTED/. NO MENTION WAS MADE OF SYMPTOMS OR COMPLAINTS, BUT THESE CONCN WERE CONSIDERED EXCESSIVE. ...
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 372]**PEER REVIEWED**

ALTHOUGH INDIVIDUAL RESPONSES OF MAN TO METHYL ALCOHOL MAY VARY CONSIDERABLY, INDUSTRIAL EXPOSURES ARE NOT VERY HAZARDOUS IF CONCN ARE MAINTAINED WITHIN UPPER LIMIT OF 200 PPM BY PROPER VENTILATION.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4541]**PEER REVIEWED**

2,062,431 Workers are potentially exposed to methanol based on statistical estimates derived from the NIOSH Survey conducted between 1972-74 in the USA(1). In a survey conducted between 1978-1982 of solvent products used in industrial workplaces and having worker exposure, methanol was identified in 9.8% of the 275 solvent samples collected by factory inspectors(2); the products represented solvent classes such as thinners, degreasers, paints, inks, and adhesives(2).
[(1) NIOSH; National Occupational Hazard Survey (NOHS) (1974) (2) Lehmann E et al; pp. 31-41 in Safety Health Aspects Org Solvents. Alan R Liss Inc (1986)]**PEER REVIEWED**

Body Burden:

Methanol was detected in 1 of 12 samples of human milk collected from volunteers in 4 USA cities(1). Methanol has been detected in expired human air(2,3,4); in one study, it was detected in 3.6% of 387 expired air samples collected from 54 volunteers at a geometric mean concn of 0.549 ng/l(4).
[(1) Pellizzari ED et al; Bull Environ Contam Toxicol 28: 322-8 (1982) (2) Krotosynski B; J Chromat Sci 15: 239-44 (1977) (3) Krotosynski BK, O'Neill HJ; J Environ Sci Health Part A-Environ Sci Eng 17: 855-83 (1982) (4) Krotosynski BK et al; J Anal Toxicol 3: 255-43 (1979)]**PEER REVIEWED**

Average Daily Intake:

AIR INTAKE: assume 1.0-25.0 ppb (0.76-19 ug/cu m)(1): 15.2-380 ug(2); WATER INTAKE: insufficient data; FOOD INTAKE: insufficient data.
[(1) Snider JR, Dawson, GA; J Geophys Res D2, 90: 3797-805 (1985) (2) Jonaaon A et al; Environ International 11: 383-92 (1985)]**PEER REVIEWED**

Minimum Fatal Dose Level:

The minimum lethal dose of methanol in the absence of medical treatment is between 0.3 and 1 g/kg.
[Environmental Health Criteria 196: Methanol pp. 8 (1997) by the International Programme on Chemical Safety (IPCS) under the joint sponsorship of the United Nations Environment Programme, the International Labour Organisation and the World Health Organization.]**QC REVIEWED**

Emergency Medical Treatment:

Emergency Medical Treatment:

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The following Overview, *** METHANOL ***, is relevant for this HSDB record chemical.

Life Support:
  o   This overview assumes that basic life support measures
      have been instituted.
Clinical Effects:
  SUMMARY OF EXPOSURE
   0.2.1.1 ACUTE EXPOSURE
     o   Methanol is highly toxic, producing metabolic acidosis,
         blindness, and death.  Evidence of metabolism and/or
         symptoms may be delayed for 18 to 24 hours (Range 1-72
         hours).  Toxicity is related to the degree of acidosis
         and thus the time between exposure and specific
         treatment.  Prognosis is poor in patients with coma or
         seizure and severe metabolic acidosis (pH <7).
     o   Toxic exposure may occur by ingestion, inhalation, or
         dermal routes.
     o   Acute poisoning causes initial drowsiness, confusion
         and ataxia.  These  symptoms are similar to mild
         ethanol inebriation.  Because methanol must be
         metabolized, additional clinical signs and laboratory
         findings of  metabolic acidosis may be delayed for 18
         to 24 hours.  Then the patient  may experience
         nonspecific malaise, headache, vomiting, abdominal
         pain, nausea, vomiting, and visual changes.  If
         untreated, CNS depression progresses to encephalopathy,
         rapid respirations, wide anion gap metabolic acidosis
         with hypokalemia.  Coma and death follow.  Visual
         defects are described as blurred or "snowfield" like
         vision.
      1.  If untreated, methanol poisoning progresses to coma,
          metabolic acidosis, and finally respiratory or
          circulatory arrest.
     o   The most common permanent sequelae following severe
         poisoning are optic neuropathy, blindness,
         Parkinsonism, toxic encephalopathy, and polyneuropathy.
  HEENT
   0.2.4.1 ACUTE EXPOSURE
     o   Blurred or double vision, constricted visual fields,
         spots before the eyes, sharply reduced visual acuity,
         optic atrophy, blindness, nystagmus, and whiteness in
         the visual field have been described.  Fixed dilated
         pupils suggest severe poisoning.
      1.  Onset of effects may be delayed for 12 to 24 hours.
          Fundoscopic findings may be normal or may show
          peripapillary edema, hyperemia of the optic disc, or
          retinal edema.
  CARDIOVASCULAR
   0.2.5.1 ACUTE EXPOSURE
     o   Tachycardia or bradycardia may develop in fatal
         poisonings.  Cardiac failure and severe hypotension may
         occur.
  RESPIRATORY
   0.2.6.1 ACUTE EXPOSURE
     o   Tachypnea from metabolic acidosis is common.  Sudden
         respiratory failure may occur in the terminal stages.
  NEUROLOGIC
   0.2.7.1 ACUTE EXPOSURE
     o   Seizures, coma, and symptoms similar to ethanol
         "hangover" may occur.
     o   Permanent sequelae may include basal ganglia infarcts,
         Parkinsonism, toxic encephalopathy, polyneuropathy,
         optic atrophy and blindness.
  GASTROINTESTINAL
   0.2.8.1 ACUTE EXPOSURE
     o   Abdominal pain, anorexia, nausea, and vomiting may
         occur.  In severe poisonings, acute necrotizing
         pancreatitis has been reported.
  GENITOURINARY
   0.2.10.1 ACUTE EXPOSURE
     o   Acute renal failure and hematuria have been reported.
  ACID-BASE
   0.2.11.1 ACUTE EXPOSURE
     o   Metabolic acidosis is classic.  Acidosis may be delayed
         for 18 to 24 hours, or longer with concurrent ethanol
         ingestion.
  FLUID-ELECTROLYTE
   0.2.12.1 ACUTE EXPOSURE
     o   Hypomagnesemia, hypokalemia, and hypophosphatemia have
         been reported.
  MUSCULOSKELETAL
   0.2.15.1 ACUTE EXPOSURE
     o   Rhabdomyolysis may occur in severe poisonings.
  REPRODUCTIVE HAZARDS
    o   Methanol, together with other solvents has been linked
        with birth defects of the central nervous system in
        humans (Holmberg, 1979), but methanol cannot be
        considered to be a human reproductive hazard because of
        mixed or poorly documented exposures.
  CARCINOGENICITY
   0.2.21.1 IARC CATEGORY
     o   At the time of this review, no studies were found on
         the possible carcinogenic activity of methanol in
         humans or experimental animals.
Laboratory:
  o   Obtain CBC, electrolytes, urinalysis, and arterial blood
      gases in symptomatic patients or those with a history of
      significant exposure.
  o   Measure serum pH and electrolytes.  A wide anion gap
      metabolic acidosis suggests the possibility of methanol
      overdose.
  o   Obtain serum methanol and ethanol levels.  If a methanol
      level cannot be obtained rapidly, an approximation may be
      calculated from the difference between a measured (using
      freezing point depression) and calculated serum
      osmolarity.  An elevated osmolal gap suggests methanol
      poisoning but a normal osmolal gap does NOT reliably
      exclude methanol poisoning.             
Treatment Overview:
  ORAL EXPOSURE
    o   Life support measures should be provided because CNS
        depression, cardiopulmonary failure, and metabolic
        acidosis have been reported with massive exposures.
    o   Because of the potential for aspiration and CNS
        depression, DO NOT induce emesis.
    o   GASTRIC ASPIRATION - Large volumes of ingested methanol
        may produce delayed gastric emptying; thus, there may be
        significant recovery of methanol even hours after
        ingestion.  Insert a nasogastric tube and aspirate
        gastric contents after significant ingestion.
    o   Significant esophageal or gastrointestinal tract
        irritation or burns may occur following ingestion.  The
        possible benefit of early removal of some ingested
        material by cautious gastric lavage must be weighed
        against potential complications of bleeding or
        perforation.
    o   ACTIVATED CHARCOAL:  Administer charcoal as a slurry
        (240 mL water/30 g charcoal).  Usual dose:  25 to 100 g
        in adults/adolescents, 25 to 50 g in children (1 to 12
        years), and 1 g/kg in infants less than 1 year old.
    o   GASTRIC LAVAGE:  Consider after ingestion of a
        potentially life-threatening amount of poison if it can
        be performed soon after ingestion (generally within 1
        hour).  Protect airway by placement in Trendelenburg and
        left lateral decubitus position or by endotracheal
        intubation.  Control any seizures first.
     1.  CONTRAINDICATIONS:  Loss of airway protective reflexes
         or decreased level of consciousness in unintubated
         patients; following ingestion of corrosives;
         hydrocarbons (high aspiration potential); patients at
         risk of hemorrhage or gastrointestinal perforation; and
         trivial or non-toxic ingestion.
    o   SEIZURES:  Administer a benzodiazepine IV; DIAZEPAM
        (ADULT:  5 to 10 mg,  repeat every 10 to 15 min as
        needed.  CHILD:  0.2 to 0.5 mg/kg, repeat every  5 min
        as needed) or LORAZEPAM (ADULT:  2 to 4 mg; CHILD:  0.05
        to 0.1 mg/kg).
     1.  Consider phenobarbital if seizures recur after diazepam
         30 mg (adults)  or 10 mg (children > 5 years).
     2.  Monitor for hypotension, dysrhythmias, respiratory
         depression, and need  for endotracheal intubation.
         Evaluate for hypoglycemia, electrolyte disturbances,
         hypoxia.
    o   ACUTE LUNG INJURY:  Maintain ventilation and oxygenation
        and evaluate with frequent arterial blood gas or pulse
        oximetry monitoring.  Early use of PEEP and mechanical
        ventilation may be needed.
    o   Monitor arterial blood gases, electrolytes, acid-base
        status, CBC (especially increased MCV), and renal
        function tests.  Monitor blood levels of methanol,
        ethanol, concomitant ingestants, and formate if
        available.
    o   ACIDOSIS - May not develop until 18 to 48 hours
        post-ingestion.  Temporize with IV sodium bicarbonate;
        monitor arterial blood gases to guide dosing.  Patients
        with metabolic acidosis need antidotal therapy (ethanol
        or fomepizole) and hemodialysis.
    o   ETHANOL THERAPY - partially inhibits the formation of
        toxic metabolites.  LOADING DOSE - 10 mL/kg of 10
        percent ethanol in D5W IV over 30 minutes.  MAINTENANCE
        DOSE - 1 to 2 mL/kg/hr of 10 percent ETHANOL in D5W by
        IV infusion.  Maintain blood ethanol levels at 100 to
        130 mg/dL.  Monitor blood glucose and blood ethanol
        levels.  INDICATIONS:  metabolic acidosis or blood
        methanol level greater than 20 mg/dL.
    o   FOMEPIZOLE - specific alcohol dehydrogenase antagonist.
        FDA approved for methanol poisoning in the USA; LOADING
        DOSE - 15 mg/kg IV over 30 minutes.  INDICATIONS - for
        methanol poisoning or suspected methanol ingestion,
        either alone or in combination with hemodialysis.
    o   HEMODIALYSIS - Maintenance ethanol dose must be
        increased during dialysis.  Fomepizole dosing should be
        increased to every 4 hr during hemodialysis.
        INDICATIONS - 1) blood methanol level greater than 50
        mg/dL (15 mmol/L); 2) severe acid-base and/or
        fluid-electrolyte abnormalities despite conventional
        therapy; 3) renal failure.
    o   LEUCOVORIN/FOLIC ACID - If symptomatic - IV leucovorin 1
        mg/kg once (up to 50 mg/dose) followed by IV folic acid
        1 mg/kg (up to 50 mg/dose) every 4 hours for 6 doses.
    o   Prognosis should be based upon the clinical
        presentation, severity of acidosis, blood methanol and
        formate levels (if available), and response to
        treatment.
  INHALATION EXPOSURE
    o   INHALATION:  Move patient to fresh air.  Monitor for
        respiratory distress.  If cough or difficulty breathing
        develops, evaluate for respiratory tract irritation,
        bronchitis, or pneumonitis.  Administer oxygen and
        assist ventilation as required.  Treat bronchospasm with
        beta2  agonist and corticosteroid aerosols.
    o   Treatment should include recommendations listed in the
        ORAL EXPOSURE section when appropriate.
  EYE EXPOSURE
    o   DECONTAMINATION:  Irrigate exposed eyes with copious
        amounts of tepid water for at least 15 minutes.  If
        irritation, pain, swelling, lacrimation, or photophobia
        persist, the patient should be seen in a health care
        facility.
  DERMAL EXPOSURE
    o   DECONTAMINATION:  Remove contaminated clothing and wash
        exposed  area thoroughly with soap and water.  A
        physician may need to  examine the area if irritation or
        pain persists.
Range of Toxicity:
  o   Serious toxicity may occur from ingestion of 0.25 mL/kg of
      100 percent methanol.  Fatalities might occur from
      ingestion of 0.5 mL/kg of 100 percent methanol.  Mortality
      is related to the time interval between exposure and the
      institution of specific therapy.

[Rumack BH: POISINDEX(R) Information System. Micromedex, Inc., Englewood, CO, 2003; CCIS Volume 116, edition exp May, 2003. Hall AH & Rumack BH (Eds):TOMES(R) Information System. Micromedex, Inc., Englewood, CO, 2003; CCIS Volume 116, edition exp May, 2003.] **PEER REVIEWED**

Antidote and Emergency Treatment:

EXPTL USE: IN RABBITS, PYRAZOLE ONLY MARGINALLY PROTECTED AGAINST METHANOL TOXICITY.
[RAO ET AL; BULL JAWAHARLAL INST POST-GRAD MED EDUC RES 2: 1-11 (1979)]**PEER REVIEWED**

Animal Toxicity Studies:

Toxicity Summary:

Methanol occurs naturally in humans, animals and plants. It is a natural constituent in blood, urine, saliva and expired air. ... The two most important sources of background body burdens for methanol and formate are diet and metabolic processes. Methanol is available in the diet principally from fresh fruits and vegetables, fruit juices ... fermented beverages ... and diet foods (principally soft drinks). The artificial sweetner aspartame is widely used and, on hydrolysis, 10% (by weight) of the molecule is converted to free methanol, which is available for absorption. ... Exposures to methanol can occur in occupational settings through inhalation or dermal contact. ... Methanol is readily absorbed by inhalation, ingestion and dermal exposure, and it is rapidly distributed to tissues according to the distribution of body water. A small amount of methanol is excreted unchanged by the lungs and kidneys. ... Methanol is metabolized primarily in the liver by sequential oxidative steps to formaldehyde, formic acid and carbon dioxide. The initial step involves oxidation to formaldehyde by hepatic alcohol dehydrogenase ... In step 2, formaldehyde is oxidized by formaldehyde dehydrogenase to formic acid/or formate depending on the pH. In step 3, formic acid is detoxified to carbon dioxide by folate-dependent reactions. Elimination of methanol from the blood via the urine and exhaled air and by metabolism appears to be slow in all species, especially when compared to ethanol. ... It is the rate of metabolic detoxification, or removal of formate that is vastly different between rodents and primates and is the basis for the dramatic differences in methanol toxicity observed between rodents and primates. The acute and short term toxicity of methanol varies greatly between different species, toxicity being highest in species with a relatively poor ability to metabolize formate. In such cases of poor metabolism of formate, fatal methanol poisoning occurs as a result of metabolic acidosis and neuronal toxicity, whereas, in animals that readily metabolize formate, consequences of CNS depression (coma, respiratory failure, etc.) are usually the cause of death. Sensitive primate species (humans and monkeys) develop increased blood formate concentrations following methanol exposure, while resistant rodents, rabbits and dogs do not. Humans and non-human primates are uniquely sensitive to the toxic effects of methanol. Overall methanol has a low acute toxicity to non-primate animals. ... In the rabbit, methanol is a moderate irritant to the eye. It was not skin sensitizing ... There is no evidence from animal studies to suggest that methanol is a carcinogen ... The inhalation of methanol by pregnant rodents throughout the period of embryogenesis induces a wide range of concentration-dependent teratogenic and embryolethal effects. Treatment-related malformations, primarily extra or rudimentary cervical ribs and urinary or cardiovascular defects, were found in fetuses of rats ... Increased incidences of exencephaly and cleft palate were found in the offspring of ... mice ... There was increased embryo/fetal death ... and an increasing incidence of full litter resorptions. Reduced fetal weight was observed ... Fetal malformations ... included neural and ocular defects, cleft palate, hydronephrosis and limb anomalies. Humans (and non-human primates) are uniquely sensitive to methanol poisoning and the toxic effects in these species are characterized by formic acidemia, metabolic acidosis, ocular toxicity, nervous system depression, blindness, coma and death. Nearly all of the available information on methanol toxicity in humans relates to the consequences of acute rather than chronic exposures. A vast majority of poisonings involving methanol have occurred from drinking adulterated beverages and from methanol-containing products. Although ingestion dominates as the most frequent route of poisoning, inhalation of high concentrations of methanol vapor and percutaneous absorption of methanolic liquids are as effective as the oral route in producing acute toxic effects. The most noted health consequences of longer term exposure to lower levels of methanol is a broad range of ocular effects. ... The toxicity is manifest if formate generation continues at a rate that exceeds its rate of metabolism. ... The minimum lethal dose of methanol in the absence of medical treatment is between 0.3 and 1 g/kg. The minimum dose causing permanent visual defects is unknown. ... Wide interindividual variability of the toxic dose is a prominent feature in acute methanol poisoning. Two important determinants of human susceptibility to methanol toxicity appear to be (1) concurrent ingestion of ethanol, which slows the entrance of methanol into the metabolic pathway, and (2) hepatic folate status, which governs the rate of formate detoxification. The symptoms and signs of methanol poisoning, which may not appear until after an asymptomatic period ... include visual disturbances, nausea, abdominal and muscle pain, dizziness, weakness and disturbances of consciousness ranging from coma to clonic seizures. Visual disturbances ... range from mild photophobia and misty or blurred vision to markedly reduced visual acuity and complete blindness. In extreme cases death results. The principal clinical feature is severe metabolic acidosis of the anion-gap type. The acidosis is largely attributed to the formic acid produced when methanol is metabolized. ... Visual disturbances of several types (blurring, constriction of the visible field, changes in color perception, and temporary or permanent blindness) have been reported in workers ... No other adverse effects of methanol have been reported in humans except minor skin and eye irritation. ... Methanol is of low toxicity to aquatic organisms, and effects due to environmental exposure to methanol are unlikely to be observed, except in the case of a spill.
[Environmental Health Criteria 196: Methanol pp. 1-9 (1997) by the International Programme on Chemical Safety (IPCS) under the joint sponsorship of the United Nations Environment Programme, the International Labour Organisation and the World Health Organization.]**QC REVIEWED**

Non-Human Toxicity Excerpts:

ITS CONSUMPTION LEADS TO ATROPHY OF THE OPTIC NERVE, CAUSING PERMANENT BLINDNESS, AND TO DEPRESSION OF CARDIAC AND VOLUNTARY MUSCLE, RESULTING IN DEATH.
[Garner's Veterinary Toxicology. 3rd ed., rev. by E.G.C. Clarke and M.L. Clarke. Baltimore: Williams and Wilkins, 1967. 285]**PEER REVIEWED**

EXPOSURE OF ANIMALS /TO METHANOL IN AIR/ ... MAY INDUCE ... INCREASED RATE OF RESPIRATION ... NERVOUS DEPRESSION FOLLOWED BY EXCITATION, IRRITATION OF MUCOUS MEMBRANES, ATAXIA, PARTIAL PARALYSIS, AGONY, PROSTRATION, CNS DEPRESSION, CONVULSIONS, DECREASE IN RECTAL TEMP, LOSS IN WT & DEATH.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4533]**PEER REVIEWED**

... THERE MAY BE IRREVERSIBLE BRAIN DAMAGE.
[Jones, L.M., et al. Veterinary Pharmacology & Therapeutics. 4th ed. Ames: Iowa State University Press, 1977. 1170]**PEER REVIEWED**

MICE EXPOSED TO AIR CONTAINING 48,000 PPM FOR 3.5-4 HR DAILY UP TO CUMULATIVE TOTAL OF 24 HR WERE IN STATE OF CNS DEPRESSION, BUT SURVIVED, WHEREAS THEY SUCCUMBED IN COMA WHEN CORRESPONDINGLY EXPOSED FOR 54 HR TO AIR CONTAINING 54,000 PPM.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4533]**PEER REVIEWED**

The pathologic changes found in the tissues of animals exposed by inhalation to methyl alcohol are quite similar to those observed in animals following ingestion of this compound. In the eyes of the dog ... hyperemia of the choroid and edema of the ocular tissue with early signs of degeneration of the ganglionic cells of the retina and nerve fibers /were found/ ... Vessels of the choroid of poisoned animals were markedly congested, the entire retina was edematous, and the ganglion cells were degenerated. ... Hemorrhage, edema, congestion, and pneumonia were observed in the lung of the various species that were exposed to vapors containing methyl alcohol. The livers and kidneys showed congestion, albuminous and fatty degeneration and fatty infiltration. Cardiac dilation and myocardial degeneration were observed in the hearts of animals. Degenerative injuries of the central nervous system have been described ... .
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4531]**PEER REVIEWED**

... NOTED IN BLOOD OF ANIMALS INHALING METHYL ALCOHOL AN INCREASE IN ERYTHROCYTES, HEMOGLOBIN & POLYMORPHONUCLEAR LEUCOCYTES.
[Browning, E. Toxicity and Metabolism of Industrial Solvents. New York: American Elsevier, 1965. 317]**PEER REVIEWED**

WITHIN 48 HR OF ADMIN SINGLE LD50 DOSE TO RATS, BRAIN ENZYME ACTIVITIES UNDERWENT CHANGES, GLYCOGEN LEVELS INCR, BRAIN LACTIC ACID LEVELS INCR 1 HR AFTER ADMIN, DECR 48 HR AFTER.
[MIKULICH SG; BIOL AKT VESHCHESTVA (MIKROELEM, VITAM DRUGIE) RASTENIEVOD ZHIVOTNOVOD MED 109-11 (1975)]**PEER REVIEWED**

METHANOL BLOOD LEVELS IN CHRONICALLY FED RABBITS INCR WITH TIME. CUMULATIVE LEVELS ALSO SEEN IN ACUTE STUDIES. BRAIN EDEMA, EYE EDEMA, & MYELIN THINNING OR LOSS WERE OBSERVED IN ACUTE & CHRONIC STUDIES.
[RAO ET AL; BULL JAWAHARLAL INST POST-GRAD MED EDUC RES 2: 1-11 (1977)]**PEER REVIEWED**

AFTER METHANOL ADMIN, MACACA MULATTA SHOWED OPTIC EDEMA, ACIDOSIS, & FORMATE ACCUMULATION IN BLOOD.
[MARTIN-AMAT ET AL; TOXICOL APPL PHARMACOL 45 (1) 201-8 (1978)]**PEER REVIEWED**

@ 40,000-80,000 MG/L, METHANOL KILLED CHIRONOMUS DORSALIS MEIG LARVAE WITHIN 2 DAYS; 500-20,000 MG/L WITHIN 26 DAYS. 50-200 MG/L DELAYED IMAGO EMERGENCE; @ EMERGENCE, LIMB DEFECTS OR HEMORRHAGES WERE OBSERVED. 250 MG/L CAUSE MORPHOLOGICAL CHANGES IN LARVAL DEVELOPMENT.
[PUZIKOVA NB; TR SARAT OTD GOSNIORKH 13: 151-3 (1975)]**PEER REVIEWED**

The effects of alcohol on audition were studied in the rat by examining the modification of acoustic startle reflexes by pure tone pulses and by gaps in white noise. Groups of rats received four injections of 0.0, 0.25, 1, and 2 g/kg of either methyl or ethyl alcohol in increasing order at 1 hr intervals. One-half hour after the administration of each dose, loudness perception or temporal acuity was measured. Blood alcohol levels (mM) for the two alcohols obtained in control animals were equivalent following the final dose. Both alcohols produced a dose dependent reduction in baseline startle amplitude that was greater during exposure to ethanol than methanol. Loudness functions associated with pulse intensity were not diminished by the alcohols, however inhibition produced by a gap in noise was reduced following the highest dose of either alcohol. These data are consistent with behavioral study results that have suggested that alcohol does not affect loudness perception, and with electrophysical experiment results which indicate that alcohol disrupts temporal relationships along the primary auditory pathway.
[Wecker JR, Ison JR; Toxicol Appl Pharm 74 (2): 258-66 (1984)]**PEER REVIEWED**

Mature male rats were examined for alterations in circulating free testosterone, luteinizing hormone and follicle stimulating hormone after inhalation of methanol vapor in a dynamic system for up to six weeks at doses ranging from 200 to 10,000 ppm. The most extensive effects were noticed after exposure to 200 ppm of methanol for 6 weeks, with serum testosterone concn being 32% of the controls. A significant change in luteinizing hormone concn after exposure to 10,000 ppm of methanol for six wk was also demonstrated while follicle stimulating and the elimination rate of testosterone from the blood (which indicates effects on the testicular synthesis of testosterone) remained unchanged throughout the experiments.
[Cameron AM et al; Arch Toxicol 7: 441-3 (1984)]**PEER REVIEWED**

Rats /were exposed/ during their entire gestation for 7 hr daily to 5000, 10,000, and 20,000 ppm. Significant increases in fetal defects occurred at the highest level. The defects involved the skeletal, cardiac, and urinary system.
[Shepard, T.H. Catalog of Teratogenic Agents. 5th ed. Baltimore, MD: The Johns Hopkins University Press, 1986. 371]**PEER REVIEWED**

The results of the skin absorption experiments were described by stating that all animals subjected to the action of any amount of methyl alcohol by skin absorption had died. The lowest lethal dose was 0.5 ml/kg for one monkey. It was reported that rabbits were far less susceptible to methyl alcohol poisoning by this route than monkeys and rats. In a study of the effects of continuous administration of methyl alcohol, a known amount was dropped onto or injected into the gauze pads 4 times/day. All such treated monkeys displayed dilated pupils within 2 hr after one such administration of 1.3 mg/kg of methyl alcohol. The minimum lethal dose was a total of 4 administrations of 0.5 ml/kg methyl alcohol in one day, and it was concluded that sufficient methyl alcohol could be absorbed through the skin to cause death and that the threshold for immediated danger in monkeys was below the minimum lethal dose.
[McCord CP; Ind Eng Chem 23: 931-6 (1931) as cited in NIOSH; Criteria Document: Methyl Alcohol p.59-60 (1976) DHEW Pub. NIOSH 76-148]**PEER REVIEWED**

A negative consensus resulted from all sister chromatid exchange tests when no exogenous metabolic activation system was used.
[Latt SA et al; Mutation Res (1) 87: 17-62 (1981)]**PEER REVIEWED**

A negative consensus resulted from both cell transformation in primary cells using limited lifetime strains and cell transformation via viral enhancement tests when no exogenous metabolic activation system was used.
[Heidelberger C et al; Mutation Res (3) 114: 283-385 (1983)]**PEER REVIEWED**

A negative consensus resulted from all Neurospera crassa tests when no exogenous metabolic activation system was used.
[Brockman HE et al; Mutation Res (2) 133: 87-134 (1984)]**PEER REVIEWED**

Ecotoxicity Values:

LC50 Pimephales promelas (fathead minnows) 29.4 g/l/96 hr, (28-29 days old), confidence limit= 28.5-30.4; Test conditions: Water temp= 25 deg C, dissolved oxygen= 7.3 mg/l, water hardness= 43.5 mg/l calcium carbonate, alkalinity= 46.6 calcium carbonate, tank volume= 6.3 l, additions= 5.71 V/D, pH= 7.66 (0.03). /Conditions of bioassay not specified/
[Brooke, L.T., D.J. Call, D.T. Geiger and C.E. Northcott (eds.). Acute Toxicities of Organic Chemicals to Fathead Minnows (Pimephales Promelas). Superior, WI: Center for LakeSuperior Environmental Studies Univ. of Wisconsin Superior, 1984. 19]**PEER REVIEWED**

Metabolism/Pharmacokinetics:

Metabolism/Metabolites:

METHANOL (LABELED WITH (14)C) IS SLOWLY METABOLIZED BY RAT & IN 2 DAYS IS EXCRETED AS CARBON DIOXIDE (65% OF DOSE), & UNCHANGED METHANOL (14%) IN EXPIRED AIR, & AS FORMATE (3%) & METHANOL (3%) IN URINE.
[Parke, D. V. The Biochemistry of Foreign Compounds. Oxford: Pergamon Press, 1968. 212]**PEER REVIEWED**

... METHANOL IS ... METABOLIZED BY PATHWAYS OF 1-CARBON METABOLISM, GIVING RISE TO METHYL GROUP OF CHOLINE, ETC. IN THE RABBIT IT MAY ALSO RESULT IN ... A SMALL AMT OF METHYLGLUCURONIDE.
[Parke, D. V. The Biochemistry of Foreign Compounds. Oxford: Pergamon Press, 1968. 213]**PEER REVIEWED**

OXIDN OF METHANOL APPEARS TO OCCUR BY COUPLED PEROXIDATIVE REACTIONS CATALYZED BY HEPATIC CATALASE, & IN RATS OCCURS @ A MUCH SLOWER RATE (25 MG/KG/HR) THAN OXIDN OF ETHANOL (175 MG/KG/HR).
[Parke, D. V. The Biochemistry of Foreign Compounds. Oxford: Pergamon Press, 1968. 213]**PEER REVIEWED**

METHYL ALCOHOL IS OXIDIZED IN BODY TO FORMALDEHYDE & FORMIC ACID. ... OXIDATION ... PROCEEDS INDEPENDENTLY OF CONCN IN BLOOD. RATE ... IS ONLY ONE SEVENTH THAT OF ETHANOL, SO THAT COMPLETE OXIDATION & EXCRETION OF METHYL ALCOHOL USUALLY REQUIRE SEVERAL DAYS. OXIDATION OCCURS MAINLY IN LIVER & KIDNEY. ... EXPT WITH ISOLATED RAT-LIVER SLICES ... EMPHASIZED ... LIVER CATALASE IN OXIDIZING METHANOL ... IN MONKEY & IN MAN ALCOHOL DEHYDROGENASE IS INVOLVED IN FIRST STEP OF OXIDN.
[Gilman, A.G., L.S.Goodman, and A. Gilman. (eds.). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 7th ed. New York: Macmillan Publishing Co., Inc., 1985. 381]**PEER REVIEWED**

SEVERAL PRIMARY ALCOHOLS ... YIELD MODERATE AMT OF MONOSULFATE ESTERS. THIS PATHWAY HAS BEEN DETECTED FOR METHANOL ... IN RATS.
[Testa, B. and P. Jenner. Drug Metabolism: Chemical & Biochemical Aspects. New York: Marcel Dekker, Inc., 1976. 188]**PEER REVIEWED**

... Asparatame is broken down in the small intestine into three moieties, aspartic acid, methanol, and phenylalanine. Acute loading studies have been performed in human beings, ... who received up to 200 mg/kg. No evidence of risk to the fetus was detected. Small elevations of blood methanol following such abuse doses of asparatame did not lead to measureable increases in blood formic acid ... the product responsible for acidosis and ocular toxicity.
[Sturtevant FM; Int J Fertil 30 (1): 85-87 (1985)]**PEER REVIEWED**

Absorption, Distribution & Excretion:

METHYL ALCOHOL IS READILY ABSORBED FROM GI & RESPIRATORY TRACTS.
[Gosselin, R.E., R.P. Smith, H.C. Hodge. Clinical Toxicology of Commercial Products. 5th ed. Baltimore: Williams and Wilkins, 1984.,p. III-275]**PEER REVIEWED**

DISTRIBUTION OF METHYL ALCOHOL WITHIN TISSUES OF DOGS EXPOSED TO 4000 & 15000 PPM IN AIR OVER PERIODS RANGING FROM 12 HR TO 5 DAYS WAS FOUND TO BE RAPID. ... HIGHEST CONCN WERE FOUND IN BLOOD, EYE FLUID, BILE, & URINE, & LOWEST IN BONE MARROW & FATTY TISSUE. ... 1-7 MG OF METHYL ALCOHOL/G OF BLOOD (100-700 MG/100 ML) WAS FOUND ... IN BLOOD OF RATS FOLLOWING ORAL ADMIN OF 4 G OF METHYL ALCOHOL/KG OF BODY WEIGHT.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4534]**PEER REVIEWED**

... UNDER ... EXPTL CONDITIONS IN MAN FOLLOWING INGESTION & INHALATION. DOSAGES OF 71-84 MG/KG ORALLY RESULTED IN BLOOD LEVELS OF 4.7-7.6 MG/100 ML ... 2-3 HR AFTERWARD. URINE/BLOOD CONCN RATIO WAS ... CONSTANT @ ABOUT 1.3. ... INHALATION OF ... 500-1000 PPM ... FOR ... 3-4 HR GAVE URINE CONCN OF ABOUT 1-3 MG/100 ML. ...
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4537]**PEER REVIEWED**

IN RABBIT ONLY 1% IS EXCRETED AS FORMIC ACID IN URINE, COMPARED WITH 20% IN DOG; INTERMEDIATE VALUE IS OBTAINED IN MAN.
[Gilman, A.G., L.S.Goodman, and A. Gilman. (eds.). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 7th ed. New York: Macmillan Publishing Co., Inc., 1985. 381]**PEER REVIEWED**

METHANOL SKIN ABSORPTION SHOWED INCR RATE IN 1ST 35 MIN OF APPLICATION, FOLLOWED BY DECR @ 35-60 MIN. BIOLOGICAL HALF-LIFE OF METHANOL ELIM IN EXPIRED AIR IS 1.5 HR AFTER EITHER ORAL OR DERMAL APPLICATION.
[DUTKIEWICZ B; INT CONGR SER- EXCERPTA MED 440 (IND ENVIRON XENOBIOTICS): 106-9 (1978)]**PEER REVIEWED**

70% OF METHYL ALCOHOL LOST BY ANIMALS WAS ELIMINATED IN EXPIRED AIR.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4535]**PEER REVIEWED**

Two human male volunteers were exposed on several different occasions to methyl alcohol vapor at concentrations of from 650 to 1,430 mg/cu m (approximately 500-1,100 ppm). Concentrations were verified by analyzing air samples collected at frequent intervals during and after exposures for methyl alcohol content. Using urinary methyl alcohol concentrations as an index of methyl alcohol absorption, it was concluded that the rate of absorption was proportional to the concentration of the vapor inhaled. Exposure to methyl alcohol vapor at a concentration of 1,430 mg/cu m (approximately 1,100 ppm) for 2 1/2 hr resulted in a urinary methyl alcohol concentration of 2.56 mg/100 ml. Exposure periods were not sufficiently long to determine whether the rate of excretion would increase to equal the rate of absorption. An exposure period of 3-4 hr was all that could be reasonably tolerated. The threshold of intoxication was calculated for these two workers as 2,800 ppm (3,670 mg/cu m) and 3,000 ppm (3,930 mg/cu m) respectively.
[Leaf G, Zatman LJ; Br J Ind Med 9: 19-31 (1952) as cited in NIOSH; Criteria Document: Methyl Alcohol p.39-40 (1976) DHEW Pub. NIOSH 76-148]**PEER REVIEWED**

Biological Half-Life:

BIOLOGICAL HALF-LIFE OF METHANOL ELIM IN EXPIRED AIR IS 1.5 HR AFTER EITHER ORAL OR DERMAL APPLICATION.
[DUTKIEWICZ B; INT CONGR SER- EXCERPTA MED 440 (IND ENVIRON XENOBIOTICS) 106-9 (1978)]**PEER REVIEWED**

... Experiments were made during the morning after /human volunteers/ had consumed 1000-1500 ml red wine (9.5% weight/volume ethanol, 100 mg/l methanol) the previous evening. The washout of methanol from the body coincided with the onset of hangover. The concentrations of ethanol and methanol in blood were determined indirectly by analysis of end-expired alveolar air. In the morning when blood-ethanol dropped below the Km of liver alcohol dehydrogenase of about 100 mg/l (2.2 mM), the disappearance half-life of ethanol was 21, 22, 18 and 15 min in 4 test subjects, respectively. The corresponding elimination half-lives of methanol were 213, 110, 133 and 142 min in these same individuals. ...
[Jones AW; Pharmacol Toxicol 60 (3): 217-20 (1987)]**PEER REVIEWED**

Mechanism of Action:

... Dinitrogen oxide inhibited the oxidation of formate generated from the metabolism of methanol resulting in the development of severe metabolic acidosis and high blood formate levels in these animals compared with air breathing monkeys administered the same dose of methanol. Treatment of dinitrogen oxide exposed monkeys with repetitive doses of methionine (100 mg/kg 10, 12, and 14 hr after methanol exposure) reversed the effects of dinitrogen oxide on formate oxidation resulting in a marked decrease in blood formate levels and an increase in the rate of (14)carbon oxide formation from methanol. Methionine treatment also reversed the development of metabolic acidosis and bicarbonate depletion observed in dinitrogen oxide exposed monkeys. Thus, hepatic methionine synthetase is important in the regulation of tetrahydrofolate dependent metabolism in the monkey and the generation of this enzyme is a major factor in determining the sensitivity of a species to methanol poisoning.
[Eells JT et al; J Pharm Exp Ther 227 (2): 349-53 (1983)]**PEER REVIEWED**

Current understanding of the metabolic mechanisms of methanol toxicity is reviewed. ... It is noted that the most severe toxicty occurs many hours following peak blood and tissue methanol concentrations so that these do not necessarily provide an accurate indication of toxicity. Individual differences are seen both in this latent period and in individual susceptibility to methanol. This susceptibility may depend on the activity of folic acid requiring metabolic reactions involved in formate metabolism, formate being an intermediate produced during methanol oxidation and responsible for many toxic effects of methanol. Studies of the characteristics of methanol poisoning in non-primates and monkeys are examined. Despite the ingestion of lethal doses of methanol, non-primates generally do not develop significant metabolic acidosis nor impairment of vision, and no consistent histopathology has been demonstrated in these species. In monkeys, results suggest that the latent period represents a period of compensated metabolic acidosis; when compensatory mechanisms are exhausted, blood pH begins to drop. Formate accumulates and produces acidosis in the methanol poisoned monkey, but not in the rat, apparently due to a slower rate of formate metabolism to carbon dioxide in the monkey. ... Studies demonstrating the role of alcohol dehydrogenase in methanol metabolism in the monkey are reported; however, the catalase/peroxidative system which participates in methanol metabolism in rats apparently does not function in the monkey. Formaldehyde and formate metabolism are also examined. The regulation of the rate of formate metabolism is governed by regulation of the hepatic tetrahydrofolate concentrations. ... Further research is needed to determine what step or process it is which places the primate at a distinct liability in the metabolic disposition of one carbon moieties.
[Tephly TR, Martin KE; Food Sci Technol 12: 111-40 (1984)]**PEER REVIEWED**

Methanol toxicity is observed in monkeys and humans but is not seen in rats or mice. The expression of methanol poisoning is related to the ability of an animal to metabolize formate to carbon dioxide. Since the rate of formate oxidation is related to hepatic tetrahydrofolate content and the activites of folate dependent enzymes, studies were designed to determine hepatic concentrations of hepatic tetrahydrofolate and activites of folate dependent enzymes of human liver and livers of species considered insensitive to methanol poisoning. An excellent correlation between hepatic tetrahydrofolate and maximal rates of formate oxidation has been observed. In human liver, levels were only 50% of those observed for rat liver and similar to those found in monkey liver. Total folate was also lower (60% decreased) in human liver than that found in rat or monkey liver. Interestingly, mouse liver contains much higher hepatic tetrahydrofolate and total folate than rat or monkey liver. This is consistent with higher formate oxidation rates in this species. A second important observation has been made. 10-Formyltetrahydrofolate dehydrogenase activity, the enzyme catalyzing the final step of formate oxidation to carbon dioxide, was markedly reduced in both monkey and human liver. Thus, two mechanisms may be operative in explaining low formate oxidation in species susceptible to methanol toxicity, low hepatic tetahydrofolate levels and reduced hepatic 10-formyltetrahydrofolate dehydrogenase activity.
[Johlin FC et al; Mol Pharmacol 31 (5): 557-61 (1987)]**PEER REVIEWED**

Interactions:

... PYRAZOLE & 3-AMINO-1,2,4-TRIAZOLE ... ARE POTENT INHIBITORS OF ALCOHOL DEHYDROGENASE & CATALASE ... BOTH ARE ACTIVE IN VIVO. ... ADMIN SEPARATELY OR TOGETHER ... /TO RATS/. FINDINGS CONFIRMED ... THAT 3-AMINO-1,2,4-TRIAZOLE ... DECR RATE OF CARBON DIOXIDE PRODUCTION. ... PYRAZOLE ... SUBSTANTIALLY REDUCED OXIDN OF METHANOL TO CARBON DIOXIDE.
[The Chemical Society. Foreign Compound Metabolism in Mammals Volume 3. London: The Chemical Society, 1975. 624]**PEER REVIEWED**

COMMON BIOCHEMICAL PATHWAY OF OXIDATION OF /ETHANOL & METHANOL/ ACCOUNTS, ALSO FOR CLINICAL OBSERVATION THAT SIMULTANEOUS ADMIN OF ETHANOL MAY AMELIORATE TOXIC SEQUELAE OF METHANOL POISONING. ... PRODUCTS OF OXIDN OF METHANOL ARE TOXIC ... THEREFORE ... POISONING IS MINIMIZED IF RATE OF OXIDN OF METHANOL IS REDUCED.
[Gilman, A.G., L.S.Goodman, and A. Gilman. (eds.). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 7th ed. New York: Macmillan Publishing Co., Inc., 1985. 381]**PEER REVIEWED**

Chronic combined exposure to methanol and carbon monoxide has been reported as a causative factor of cerebral atherosclerosis.
[International Labour Office. Encyclopedia of Occupational Health and Safety. Vols. I&II. Geneva, Switzerland: International Labour Office, 1983. 1357]**PEER REVIEWED**

Pharmacology:

Interactions:

... PYRAZOLE & 3-AMINO-1,2,4-TRIAZOLE ... ARE POTENT INHIBITORS OF ALCOHOL DEHYDROGENASE & CATALASE ... BOTH ARE ACTIVE IN VIVO. ... ADMIN SEPARATELY OR TOGETHER ... /TO RATS/. FINDINGS CONFIRMED ... THAT 3-AMINO-1,2,4-TRIAZOLE ... DECR RATE OF CARBON DIOXIDE PRODUCTION. ... PYRAZOLE ... SUBSTANTIALLY REDUCED OXIDN OF METHANOL TO CARBON DIOXIDE.
[The Chemical Society. Foreign Compound Metabolism in Mammals Volume 3. London: The Chemical Society, 1975. 624]**PEER REVIEWED**

COMMON BIOCHEMICAL PATHWAY OF OXIDATION OF /ETHANOL & METHANOL/ ACCOUNTS, ALSO FOR CLINICAL OBSERVATION THAT SIMULTANEOUS ADMIN OF ETHANOL MAY AMELIORATE TOXIC SEQUELAE OF METHANOL POISONING. ... PRODUCTS OF OXIDN OF METHANOL ARE TOXIC ... THEREFORE ... POISONING IS MINIMIZED IF RATE OF OXIDN OF METHANOL IS REDUCED.
[Gilman, A.G., L.S.Goodman, and A. Gilman. (eds.). Goodman and Gilman's The Pharmacological Basis of Therapeutics. 7th ed. New York: Macmillan Publishing Co., Inc., 1985. 381]**PEER REVIEWED**

Chronic combined exposure to methanol and carbon monoxide has been reported as a causative factor of cerebral atherosclerosis.
[International Labour Office. Encyclopedia of Occupational Health and Safety. Vols. I&II. Geneva, Switzerland: International Labour Office, 1983. 1357]**PEER REVIEWED**

Minimum Fatal Dose Level:

The minimum lethal dose of methanol in the absence of medical treatment is between 0.3 and 1 g/kg.
[Environmental Health Criteria 196: Methanol pp. 8 (1997) by the International Programme on Chemical Safety (IPCS) under the joint sponsorship of the United Nations Environment Programme, the International Labour Organisation and the World Health Organization.]**QC REVIEWED**

Environmental Fate & Exposure:

Environmental Fate/Exposure Summary:

Methanol has been identified as a natural emission product from various plants and as a biological decomposition product of biological wastes and sewage. The largest anthropogenic source of methanol release to the environment is evaporation from solvent uses (1.1 billion lb/yr). If released to the atmosphere, methanol degrades via reaction with photochemically produced hydroxyl radicals with an approximate half-life of 17.8 days. Physical removal from air can occur via rainfall. If released to water, decomposition via biodegradation is expected to occur. If released to soil, methanol is expected to degrade via biodegradation and be susceptible to significant leaching. Relatively rapid evaporation from dry surfaces is likely to occur. Occupational and general exposure occurs through inhalation and dermal contact. Exposure also occurs through consumption of various foods and waters. (SRC)
**PEER REVIEWED**

Probable Routes of Human Exposure:

... eye contact
[Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) PublicationNo. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981. 1]**PEER REVIEWED**

The general population is exposed to methanol through inhalation of air, through consumption of various drinking waters and foods, and through dermal contact of various consumer products such as paint thinners and strippers, adhesives, cleaners, and inks. Widespread occupational exposure occurs through inhalation and dermal contact. (SRC)
**PEER REVIEWED**

STUDY OF WOOD HEEL INDUSTRY IN MA SHOWED AVG METHANOL VAPOR CONCN RANGING FROM 160-170 PPM, WITH NO DEFINITE EVIDENCE OF INJURY TO EXPOSED WORKERS ... CONCN BETWEEN 400 & 1000 PPM IN SPIRIT DUPLICATING PROCESSES /WERE REPORTED/. NO MENTION WAS MADE OF SYMPTOMS OR COMPLAINTS, BUT THESE CONCN WERE CONSIDERED EXCESSIVE. ...
[American Conference of Governmental Industrial Hygienists. Documentation of the Threshold Limit Values and Biological Exposure Indices. 5th ed. Cincinnati, OH:American Conference of Governmental Industrial Hygienists, 1986. 372]**PEER REVIEWED**

ALTHOUGH INDIVIDUAL RESPONSES OF MAN TO METHYL ALCOHOL MAY VARY CONSIDERABLY, INDUSTRIAL EXPOSURES ARE NOT VERY HAZARDOUS IF CONCN ARE MAINTAINED WITHIN UPPER LIMIT OF 200 PPM BY PROPER VENTILATION.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4541]**PEER REVIEWED**

2,062,431 Workers are potentially exposed to methanol based on statistical estimates derived from the NIOSH Survey conducted between 1972-74 in the USA(1). In a survey conducted between 1978-1982 of solvent products used in industrial workplaces and having worker exposure, methanol was identified in 9.8% of the 275 solvent samples collected by factory inspectors(2); the products represented solvent classes such as thinners, degreasers, paints, inks, and adhesives(2).
[(1) NIOSH; National Occupational Hazard Survey (NOHS) (1974) (2) Lehmann E et al; pp. 31-41 in Safety Health Aspects Org Solvents. Alan R Liss Inc (1986)]**PEER REVIEWED**

Body Burden:

Methanol was detected in 1 of 12 samples of human milk collected from volunteers in 4 USA cities(1). Methanol has been detected in expired human air(2,3,4); in one study, it was detected in 3.6% of 387 expired air samples collected from 54 volunteers at a geometric mean concn of 0.549 ng/l(4).
[(1) Pellizzari ED et al; Bull Environ Contam Toxicol 28: 322-8 (1982) (2) Krotosynski B; J Chromat Sci 15: 239-44 (1977) (3) Krotosynski BK, O'Neill HJ; J Environ Sci Health Part A-Environ Sci Eng 17: 855-83 (1982) (4) Krotosynski BK et al; J Anal Toxicol 3: 255-43 (1979)]**PEER REVIEWED**

Average Daily Intake:

AIR INTAKE: assume 1.0-25.0 ppb (0.76-19 ug/cu m)(1): 15.2-380 ug(2); WATER INTAKE: insufficient data; FOOD INTAKE: insufficient data.
[(1) Snider JR, Dawson, GA; J Geophys Res D2, 90: 3797-805 (1985) (2) Jonaaon A et al; Environ International 11: 383-92 (1985)]**PEER REVIEWED**

Natural Pollution Sources:

Methanol is found in wood.
[Verschueren, K. Handbook of Environmental Data of Organic Chemicals. 2nd ed. New York, NY: Van Nostrand Reinhold Co., 1983. 430]**PEER REVIEWED**

A small amount of methanol is found in the expired breath of normal subjects, possibly by endogenous metabolic production.
[Haddad, L.M. and Winchester, J.F. Clinical Management of Poisoning and Drug Overdosage. Philadelphia, PA: W.B. Saunders Co., 1983. 393]**PEER REVIEWED**

Methanol has been identified as a volatile emission product from evergreen cypress trees(1). Methanol is formed during biological decomposition of biological wastes, sewage, and sludges(2). Natural emission sources include volcanic gases, vegetation, microbes, and insects(3).
[(1) Isidorov VA et al; Atmos Environ 19: 1-8 (1985) (2) Abrams EF et al; Identification of Organic Compounds in Effluents from Industrial Sources EPA- 560/3-75-002 p. 102 (1975) (3) Graedel TE et al; Atmospheric Chemical Compounds. Sources, Occurrence, and Bioassay. Orlando, FL: Academic Press p. 232 (1986)]**PEER REVIEWED**

Some distilled fruit spirits contain, normally, high quantities of methanol. ...
[Bindler F et al; Food Addit Contam 5 (3): 343-51 (1988)]**PEER REVIEWED**

Artificial Pollution Sources:

The largest anthropogenic source of methanol release to the environment is evaporation from solvent uses which amounts to an estimated 1.1 billion lb annually(1). Annual emission releases from methanol production, end-product mfg, and storage/handling have been estimated to be 68, 49, and 12 million lb, respectively(1). Methanol is emitted in exhaust from gasoline and diesel engines(2). Other artificial sources include combustion of biomass, refuse and plastics; manufacture of petroleum, charcoal, plastics, and starch; rendering; and wood pulping(3).
[(1) USEPA; Chemical Hazard Information Profiles (CHIPS). USEPA-560/11-80-011 p. 196-7 (1980) (2) Jonsson A et al; Environ International 11: 383-92 (1985) (3) Graedel TE et al; Atmospheric Chemical Compounds. Sources, Occurrence, and Bioassay. Orlando, FL: Academic Press p. 232 (1986)]**PEER REVIEWED**

Air pollution from gasoline blended with methanol.
[Gabele PA; Proc APCA Annu Meet 80 (1): 1-24 (1987)]**PEER REVIEWED**

Environmental Fate:

TERRESTRIAL FATE: Methanol is expected to be biodegradable in soil based on the results of a large number of biological screening studies, which include soil microcosm studies. Its miscibility in water and log Kow (-0.77) suggest high mobility in soil. Based on a vapor pressure of 92 mm Hg at 20 deg C(1), evaporation from dry surfaces can be expected to occur(SRC).
[(1) Weber RC et al; Vapor Pressure Distribution of Selected Organic Chemicals. USEPA-600/2-81-021 p. 24 (1981)]**PEER REVIEWED**

AQUATIC FATE: The important environmental fate process for methanol in water is biodegradation. A large number of screening studies have found methanol to be significantly biodegradable. Volatilization half-lifes of 4.8 days and 51.7 days have been estimated for a model river (1 m deep) and an environmental pond, respectively. Aquatic hydrolysis, oxidation, photolysis, adsorption to sediment, and bioconcentration are not significant. (SRC)
**PEER REVIEWED**

ATMOSPHERIC FATE: Methanol is expected to exist almost entirely in the vapor-phase in the ambient atmosphere, based on a vapor pressure of 92 mm Hg at 20 deg C(1,2,SRC). It is degraded by reaction with photochemically produced hydroxyl radicals with an estimated half-life of 17.8 days in a typical ambient atmosphere. Atmospheric methanol can also react with nitrogen dioxide in polluted air to yield methyl nitrite. Because of methanol's water solubility, rain would be expected to physically remove some from the air(3); the detection of methanol in a thunderstorm water tends to confirm this supposition(SRC).
[(1) Weber RC et al; Vapor Pressure Distribution of Selected Organic Chemicals. USEPA-600/2-81-021 p. 24 (1981) (2) Eisenreich SJ et al; Environ Sci Technol 15: 30-8 (1981) (3) USEPA; Chemical Hazard Information Profiles (CHIPS). USEPA-560/11-80-011 p. 196-7 (1980)]**PEER REVIEWED**

Environmental Biodegradation:

Biological oxygen demand: 0.6 to 1.12 lb/lb in 5 days
[U.S. Coast Guard, Department of Transportation. CHRIS - Hazardous Chemical Data. Volume II. Washington, D.C.: U.S. Government Printing Office, 1984-5.]**PEER REVIEWED**

Standard dilution BOD water, 5-day 48% BOD Theoretical, sewage inocula(1). Warburg respirometer, 2-day 93% BOD Theoretical, activated sludge inocula(2). Warburg respirometer, 1-day 21% BOD Theoretical, activated sludge inocula(3). Standard dilution BOD water, 5-day 53.4% BOD Theoretical, 50-day 97.7% BOD Theoretical, sewage inocula(4). Warburg respirometer, 0.96-day 55% BOD Theoretical, activated sludge inocula acclimated to methanol(5). Standard dilution BOD water, 5-day 76% BOD Theoretical, 20-day 97% BOD Theoretical, sewage inocula(6). Respirometric dilution, 5-day 82.9% BOD Theoretical, sewage inocula(7). Sewage die-away, 0.4 day half-life, sewage inocula(8). Anaerobic-water, 75-80% degradation, sewage inocula(9). Biological treatment simulation, 80% degradation, adapted activated sludge(10).
[(1) Dore M et al; Trib Cebedeau 28: 3-11 (1975) (2) Gellman I, Heukelekian H; Sew Indust Wastes 27: 793-801 (1955) (3) Gerhold RM, Malaney GW; J Water Pollut Control Fed 38: 562-79 (1966) (4) Lamb CB, Jenkins GF; Proc 8th Industrial Waste Conf,. Purdue Univ p. 326-9 (1952) (5) McKinney RE, Jeris JS; Sew Indust Wastes 27: 728-35 (1955) (6) Price KS et al; J Water Pollut Control Fed 46: 63-77 (1974) (7) Wagner R; Vom Wasser 42: 271-305 (1974) (8) Wagner R; Vom Wasser 47: 241-65 (1976) (9) Bekes J et al; Proc Hung 15th Annu Meet Biochem p. 27-8 (1975) (10) Swain HM, Somerville HJ; J Appl Bacteriol 45: 147-51 (1978)]**PEER REVIEWED**

Anaerobic-water die-away, marinewater and sediment from the San Francisco Bay inocula, 3-day incubation, 83-91% degradation(1). Standard dilution, 5-day 88.7% BOD Theoretical; seawater dilution, 5-day 70.7% BOD Theoretical(2). Significant biodegradation of organic waste (methanol and acetic acid and formic acid) observed when injected into wells (850-1000 ft depth) as determined by concn monitoring and microbial population count(3). Methanol found to be susceptible to biodegradation in subsurface regions in microcosm studies simulating subsurface conditions; complete degradation within one year or less(4). Methanol degraded readily in test tube microcosms simulating subsurface soils and groundwaters from sites in VA and NY(5). Soil-sediment suspensions, aerobic conditions, 5-day CO2 evolution (14-C) of 53.4%(6); soil-sediment suspensions, anaerobic conditions, 5-day CO2 evolution (14-C) of 46.3%(6).
[(1) Oremland RL et al; Nature 296: 143-5 (1982) (2) Takemoto S et al; Suishitsu Odaku Kenkyu 4: 80-90 (1981) (3) Ditommaso A, Elkan GH; Underground Waste Manage Artif Recharge, Prepr Pap Int Symp, 2nd 1: 585-99 (1973) (4) Novak JT et al; Wat Sci Tech 17: 71-85 (1985) (5) Goldsmith CD; Diss Abstr Int B 46: 3767 (1985) (6) Scheunert I et al; Chemosphere 16: 1031-41 (1987)]**PEER REVIEWED**

Environmental Abiotic Degradation:

The experimentally recommended rate constant for the vapor-phase reaction of methanol with photochemically produced hydroxyl radicals has been reported to be 0.9X10-12 cu cm/molecule-sec at 25 deg C(1); the atmospheric half-life for this reaction can be estimated to be 17.8 days, assuming an average atmospheric hydroxyl radical concn of 5X10+5 molecules/cu cm(1,SRC). Formaldehyde is formed from the reaction of methanol with hydroxyl radicals in the atmosphere(1). The reaction of methanol with nitrogen dioxide may be the major source of methyl nitrite found in polluted atmospheres(2).
[(1) Atkinson RA; Chem Rev 85: 60-201 (1985) (2) Takagi H et al; Environ Sci Technol 20: 387-93 (1986)]**PEER REVIEWED**

The rate constant for the reaction of methanol with hydroxyl radicals in aqueous solution is approximately 1X10+9 l/mol-sec(1); if the hydroxyl radical concn of sunlit natural water is assumed to be 1X10-17 moles/l(2), the half-life would be approximately 2.2 years(SRC). Methanol in aqueous solution exhibited no degradation when exposed to sunlight using an EPA test protocol(3). Sediment and clay suspensions solution did not photocatalyze the degradation of methanol in aqueous solution during irradiation with uv light(4). Alcohols are generally resistant to environmental aqueous hydrolysis(5).
[(1) Guesten H et al; Atmos Environ 15: 1763-5 (1981) (2) Mill T et al; Science 207: 886-7 (1980) (3) Hustert K et al; Chemosphere 10: 995-8 (1981) (4) Oliver BG et al; Environ Sci Technol 13: 1075-7 (1979) (5) Lyman WJ et al; Handbook of Chemical Property Estimation Methods NY: McGraw-Hill p. 7-4 (1982)]**PEER REVIEWED**

Environmental Bioconcentration:

The biconcentration factor of methanol experimentally measured in fish (golden ide) was less than 10(1). Based on a log Kow of -0.77(2), the BCF value for methanol can be estimated to be 0.2 from a recommended regression-derived equation(3,SRC).
[(1) Freitag D et al; Chemosphere 14: 1589-1616 (1985) (2) Hansch C, Leo AJ; Medchem Project Issue No 26. Clarmont CA: Pomona College (1985) (3) Lyman WJ et al; Handbook of Chemical Property Estimation Methods NY: McGraw-Hill p. 5-4 (1982)]**PEER REVIEWED**

Soil Adsorption/Mobility:

Methanol is completely miscible in water and has a log Kow of -0.77(1,2). These properties are indicative of high mobility in soil(SRC).
[(1) Merck Index; An Encyclopedia of Chemicals, Drugs and Biologicals 10th ed p. 853 (1983) (2) Hansch C, Leo AJ; Medchem Project Issue No. 26 Claremont CA: Pomona College (1985)]**PEER REVIEWED**

Volatilization from Water/Soil:

Methanol has an y measured Henry's Law Constant of 4.4X10-6 atm-cu m/mole at 25 ). This value of Henry's Law Constant indicates that volatilization from environmental waters may be significant(2). The volatilization half-life from a iver (1 meter deep flowing 1 m/sec with a wind speed of 3 m/sec) has been be 4.8 days(2,SRC). The volatilization half-life from model pond has been estimated to be 51.7 days(3,SRC).
[(1) Snider JR, Dawson GA; J Geophys Res, D2, 90: 3797-805 (1985) (2) Lyman WJ (1982) (3) USEPA; EXAMS II Computer Simulation (1987)]**PEER REVIEWED**

Environmental Water Concentrations:

DRINKING WATER: Methanol has been qualitatively detected in drinking water from Miami, FL, Seattle, WA, Philadelphia, PA, Cincinnati, OH, and New Orleans, LA(1,2). As part of the USEPA National Organics Reconnaissance Survey (NORS), methanol was detected in 6 of 10 drinking waters from USA cities(3).
[(1) USEPA; Preliminary Assessment of Suspected Carcinogens in Water. Interim Report to Congress, June, 1975. Washington DC (1975) (2) USEPA; New Orleans Area Water Supply Draft Analytical Report by the Lower Mississippi River Facility, Sliddell, LA. Dallas, TX (1974) (3) Bedding ND et al; Sci Total Environ 25: 143-67 (1982)]**PEER REVIEWED**

RAIN WATER: Methanol was detected at a mean level of 22 ppb in thunderstorm water collected from Santa Rita, AZ in Sept, 1982(1).
[(1) Snider JR, Dawson GA; J Geophys Res, D2, 90: 3797-805 (1985)]**PEER REVIEWED**

Effluent Concentrations:

Methanol levels of 18-70 ppm were detected in wastewater effluents from a chemical mfg facility (near the Brackish River), but none was detected in associated river water or sediments(1). Methanol has been identified in wastewater effluents from chemical, paper, and latex manufacturing plants and from sewage treatment plants(2). Concn of 42.4 ppm detected in leachate from the Love Canal in Niagara Falls, NY(3). Concn of 1050 ppm detected in condensate waters from a coal-gasification plant(4). Levels of 0.1-0.6 ppm were found in exhausts from engines using simple hydrocarbon fuels(5). Methanol has been identified in exhausts from both gasoline and diesel engines(6).
[(1) Jungclaus GA et al; Environ Sci Technol 12: 88-96 (1978) (2) Shackelford WM, Keith LH; Frequency of Organic Compounds Identified in Water EPA-600/4-76- 062 p. 169 (1976) (3) Venkataramani ES et al; CRC Crit Rev Environ Control 14: 333-76 (1984) (4) Mohr DH, King J; Environ Sci Technol 19: 929-35 (1985) (5) Seizinger DE, Dimitriades B; J Air Pollut Control Assoc 22: 47-51 (1972) (6) Jonsson A et al; Environ International 11: 383-92 (1985)]**PEER REVIEWED**

Atmospheric Concentrations:

Methanol was detected at mean ambient atmospheric concn of 7.9 and 2.6 ppb at two remote AZ locations during 1982 monitoring(1). Concns of 0.0-1.2 ppb (ave 0.77 ppb methanol and ethanol) were identified in arctic air from Point Barrows, Alaska in Sept 1967(2). Avg ambient concn of 3.83-26.7 ppb detected at 5 sites in and around Stockholm, Sweden(3). Methanol has been detected (concn not reported) in indoor air of residential and office buildings(4,5).
[(1) Snider JR, Dawson GA; J Geophys Res, D2, 90: 3797-805 (1985) (2) Cavanagh LA et al; Environ Sci Technol 3: 251-7 (1969) (3) Jonsson A et al; Environ International 11: 383-92 (1985) (4) Jarke FH et al; ASHRAE Trans 87: 153-66 (1981) (5) Tsuchiya Y; Volatile Organic Compounds in Indoor Air. Am Chem Soc Div Environ Chem Preprint, New Orleans, LA 27: 183-5 (1987)]**PEER REVIEWED**

Food Survey Values:

Methanol has been identified as a volatile component of dried legumes (concn 1.5-7.9 ppm), baked potatoes, and roasted filbert nuts(1,2,3).
[(1) Coleman EC et al; J Agric Food Chem 29: 42-8 (1981) (2) Kinlin TE et al; J Agric Food Chem 20: 1021-8 (1972) (3) Lovegren NV et al; J Agric Food Chem 27: 851-3 (1979)]**PEER REVIEWED**

Plant Concentrations:

Methanol has been identified as a volatile emission product from evergreen cypress trees(1) and alfalfa(2).
[(1) Isidorov VA et al; Atmos Environ 19: 1-8 (1985) (2) Owens LD et al; Phytopathology 59: 1468-72 (1969)]**PEER REVIEWED**

Other Environmental Concentrations:

Methanol was identified as a component of several industrial paint strippers(1). Engine exhausts from both gasoline and diesel vehicles have been found to contain methanol(2). Methanol has been identified as a constituent of tobacco smoke(3).
[(1) Hahn WJ, Werschulz PO; Evaluation of Alternatives to Toxic Organic Paint Strippers. USEPA-600/S2-86-063 (1986) (2) Jonsson A et al; Environ International 11: 383-92 (1985) (3) Graedel TE et al; Atmospheric Chemical Compounds. Sources, Occurrence, and Bioassay. Orlando, FL: Academic Press p. 232 (1986)]**PEER REVIEWED**

Environmental Standards & Regulations:

FIFRA Requirements:

Unless designated as an active ingredient in accordance with paragraph (b) or (c) of this section, this substance, when used in antimicrobial products, is considered inert, having no independent pesticidal activity. The percentage of such an ingredient shall be included on the label in the total percentage of inert ingredients.
[40 CFR 162.60 (7/1/88)]**PEER REVIEWED**

Methyl alcohol is exempted from the requirement of a tolerance when used in accordance with good agricultural practice as inert (or occasionally active) ingredients in pesticide formulations applied to growing crops only.
[40 CFR 180.1001 (7/1/88)]**PEER REVIEWED**

Methyl alcohol is exempted from the requirement of a tolerance when used in accordance with good agricultural practice as inert (or occasionally active) ingredients in pesticide formulations applied to animals.
[40 CFR 180.1001 (7/1/88)]**PEER REVIEWED**

Residues of methyl alcohol are exempted from the requirement of a tolerance when used in accordance with good agricultural practice as inert (or occasionally active) ingredients in pesticide formulations applied to growing crops or to raw agricultural commodities after harvest.
[40 CFR 180.1001 (7/1/88)]**PEER REVIEWED**

CERCLA Reportable Quantities:

Persons in charge of vessels or facilities are required to notify the National Response Center (NRC) immediately, when there is a release of this designated hazardous substance, in an amount equal to or greater than its reportable quantity of 5000 lb or 2270 kg. The toll free telephone number of the NRC is (800) 424-8802; in the Washington metropolitan area (202) 426-2675. The rule for determining when notification is required is stated in 40 CFR 302.6.
[40 CFR 302.4 (7/1/88)]**PEER REVIEWED**

RCRA Requirements:

As stipulated in 40 CFR 261.33, when methanol, as a commercial chemical product or manufacturing chemical intermediate or an off-specification commercial chemical product or a manufacturing chemical intermediate, becomes a waste, it must be managed according to Federal and/or State hazardous waste regulations. Also defined as a hazardous waste is any residue, contaminated soil, water, or other debris resulting from the cleanup of a spill, into water or on dry land, of this waste. Generators of small quantities of this waste may qualify for partial exclusion from hazardous waste regulations (40 CFR 261.5).
[40 CFR 261.33 (7/1/88)]**PEER REVIEWED**

When methanol is a spent solvent, it is classified as a hazardous waste from a nonspecific source (F003), as stated in 40 CFR 261.31, and must be managed according to State and/or Federal hazardous waste regulations.
[40 CFR 261.31 (7/1/88)]**PEER REVIEWED**

Atmospheric Standards:

This action promulgates standards of performance for equipment leaks of Volatile Organic Compounds (VOC) in the Synthetic Organic Chemical Manufacturing Industry (SOCMI). The intended effect of these standards is to require all newly constructed, modified, and reconstructed SOCMI process units to use the best demonstrated system of continuous emission reduction for equipment leaks of VOC, considering costs, non air quality health and environmental impact and energy requirements. Methanol is produced, as an intermediate or final product, by process units covered under this subpart.
[40 CFR 60.489 (7/1/87)]**PEER REVIEWED**

Listed as a hazardous air pollutant (HAP) generally known or suspected to cause serious health problems. The Clean Air Act, as amended in 1990, directs EPA to set standards requiring major sources to sharply reduce routine emissions of toxic pollutants. EPA is required to establish and phase in specific performance based standards for all air emission sources that emit one or more of the listed pollutants. Methanol is included on this list.
[Clean Air Act as amended in 1990, Sect. 112 (b) (1) Public Law 101-549 Nov. 15, 1990]**QC REVIEWED**

State Drinking Water Guidelines:

(FL) FLORIDA 5,000 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(MN) MINNESOTA 3000 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

(WI) WISCONSIN 5000 ug/l
[USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93)] **QC REVIEWED**

FDA Requirements:

Methyl alcohol is an indirect food additive for use only as a component of adhesives.
[21 CFR 175.105 (4/1/88)]**PEER REVIEWED**

Chemical/Physical Properties:

Molecular Formula:

C-H4-O
**PEER REVIEWED**

Molecular Weight:

32.04
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Color/Form:

Colorless liquid
[NIOSH. NIOSH Pocket Guide to Chemical Hazards. DHHS (NIOSH) Publication No. 94-116. Washington, D.C.: U.S. Government Printing Office, June 1994. 200]**QC REVIEWED**

Odor:

ALCOHOLIC ODOR; PUNGENT ODOR WHEN CRUDE
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Characteristic pungent odor.
[NIOSH. NIOSH Pocket Guide to Chemical Hazards. DHHS (NIOSH) Publication No. 94-116. Washington, D.C.: U.S. Government Printing Office, June 1994. 200]**QC REVIEWED**

Boiling Point:

64.7 DEG C @ 760 MM HG
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Melting Point:

-97.8 DEG C
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Critical Temperature & Pressure:

CRITICAL TEMP: 240.0 DEG C; CRITICAL PRESSURE: 78.5 ATM
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Density/Specific Gravity:

0.8100 @ 0 DEG C/4 DEG C
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Dissociation Constants:

pKa = 15.3
[Serjeant EP, Dempsey B; Ionisation constants of organic acids in aqueous solution. IUPAC Chem Data Ser No.23. NY,NY: Pergamon pp.989 (1979)]**QC REVIEWED**

Heat of Combustion:

723 KJ/mole
[Environment Canada; Tech Info for Problem Spills: Methanol (Draft) p.4 (1981)]**PEER REVIEWED**

Heat of Vaporization:

39.2 KJ/mole
[Environment Canada; Tech Info for Problem Spills: Methanol (Draft) p.4 (1981)]**PEER REVIEWED**

Octanol/Water Partition Coefficient:

log Kow= -0.77
[Hansch, C., Leo, A., D. Hoekman. Exploring QSAR - Hydrophobic, Electronic, and Steric Constants. Washington, DC: American Chemical Society., 1995. 3]**QC REVIEWED**

Solubilities:

MISCIBLE WITH ETHANOL, ETHER, BENZENE, MOST ORGANIC SOLVENTS AND KETONES.
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Sol in acetone, chloroform
[Weast, R.C. (ed.) Handbook of Chemistry and Physics. 69th ed. Boca Raton, FL: CRC Press Inc., 1988-1989.,p. C-672]**PEER REVIEWED**

Completely miscible in water @ 20 deg C
[Flick, E.W. Industrial Solvents Handbook. 3rd ed. Park Ridge, NJ: Noyes Publications, 1985. 188]**PEER REVIEWED**

Water solubility = miscible
[Riddick, J.A., W.B. Bunger, Sakano T.K. Techniques of Chemistry 4th ed., Volume II. Organic Solvents. New York, NY: John Wiley and Sons., 1985. 190]**QC REVIEWED**

Spectral Properties:

INDEX OF REFRACTION: 1.3292 @ 20 DEG C/D
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

MAX ABSORPTION (GAS): 183.3 NM (LOG E= 2.18)
[Weast, R.C. (ed.). Handbook of Chemistry and Physics. 52nd ed. Cleveland: The Chemical Rubber Co., 1972.,p. C-370]**PEER REVIEWED**

IR: 287 (Sadtler Research Laboratories IR Grating Collection)
[Weast, R.C. and M.J. Astle. CRC Handbook of Data on Organic Compounds. Volumes I and II. Boca Raton, FL: CRC Press Inc. 1985.,p. V1 844]**PEER REVIEWED**

UV: 1-3 (Organic Electronic Spectral Data, Phillips et al, John Wiley & Sons, New York)
[Weast, R.C. and M.J. Astle. CRC Handbook of Data on Organic Compounds. Volumes I and II. Boca Raton, FL: CRC Press Inc. 1985.,p. V1 844]**PEER REVIEWED**

NMR: 1 (Varian Associates NMR Spectra Catalogue)
[Weast, R.C. and M.J. Astle. CRC Handbook of Data on Organic Compounds. Volumes I and II. Boca Raton, FL: CRC Press Inc. 1985.,p. V1 844]**PEER REVIEWED**

MASS: 6 (Atlas of Mass Spectral Data, John Wiley & Sons, New York)
[Weast, R.C. and M.J. Astle. CRC Handbook of Data on Organic Compounds. Volumes I and II. Boca Raton, FL: CRC Press Inc. 1985.,p. V1 844]**PEER REVIEWED**

Surface Tension:

22.61 mN/m (at 20 deg C)
[Environment Canada; Tech Info for Problem Spills: Methanol (Draft) p.4 (1981)]**PEER REVIEWED**

Vapor Density:

1.11
[Environment Canada; Tech Info for Problem Spills: Methanol (Draft) p.3 (1981)]**PEER REVIEWED**

Vapor Pressure:

127 mm Hg at 25 deg C
[Boublik T et al; The vapor pressures of pure substances: selected values of the temperature dependence of the vapour pressures of some pure substances in the normal and low pressure region. Vol. 17. Amsterdam, Netherlands: Elsevier Sci. Publ (1984)]**QC REVIEWED**

Viscosity:

0.614 mPa sec
[Environment Canada; Tech Info for Problem Spills: Methanol (Draft) p.4 (1981)]**PEER REVIEWED**

Other Chemical/Physical Properties:

DIPOLE MOMENT: 1.69; SPECIFIC HEAT: 0.595-0.605 AT 20-25 DEG C, FORMS AZEOTROPES WITH MANY CMPD; BURNS WITH NONLUMINOUS BLUISH FLAME ...
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

1 MG/L= 764 PPM; 1 PPM= 1.31 MG/CU M @ 25 DEG C, 760 MM HG
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4530]**PEER REVIEWED**

Heat of fusion: 23.70 cal/g
[Weast, R.C. (ed.) Handbook of Chemistry and Physics. 69th ed. Boca Raton, FL: CRC Press Inc., 1988-1989.,p. C-666]**PEER REVIEWED**

Partition coefficients at 37 deg C for methanol into blood= 2,100; into oil= 56.
[Sato A, Nakajima T; Scand J Work Environ Health 13: 81-93 (1987)]**PEER REVIEWED**

VAPOR PRESSURE= 100 MM HG @ 21.2 DEG C
[Sax, N.I. Dangerous Properties of Industrial Materials. 6th ed. New York, NY: Van Nostrand Reinhold, 1984. 1764]**QC REVIEWED**

Henry's Law constant = 4.55X10-6 atm-cu m/mol at 25 deg C
[Gaffney JS et al; Environ Sci Technol 21: 519-23 (1987)]**QC REVIEWED**

Chemical Safety & Handling:

DOT Emergency Guidelines:

Health: Toxic; may be fatal if inhaled, ingested or absorbed through skin. Inhalation or contact with some of these materials will irritate or burn skin and eyes. Fire will produce irritating, corrosive and/or toxic gases. Vapors may cause dizziness or suffocation. Runoff from fire control or dilution water may cause pollution.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

Fire or explosion: Highly flammable: Will be easily ignited by heat, sparks or flames. Vapors may form explosive mixtures with air. Vapors may travel to source of ignition and flash back. Most vapors are heavier than air. They will spread along ground and collect in low or confined areas (sewers, basements, tanks). Vapor explosion and poison hazard indoors, outdoors or in sewers. Those substances designated with a "P" may polymerize explosively when heated or involved in a fire. Runoff to sewer may create fire or explosion hazard. Containers may explode when heated. Many liquids are lighter than water.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

Public safety: Call Emergency Response Telephone Number. ... Isolate spill or leak area immediately for at least 100 to 200 meters (330 to 660 feet) in all directions. Keep unauthorized personnel away. Stay upwind. Keep out of low areas. Ventilate closed spaces before entering.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

Protective clothing: Wear positive pressure self-contained breathing apparatus (SCBA). Wear chemical protective clothing which is specifically recommended by the manufacturer. It may provide little or no thermal protection. Structural firefighters' protective clothing provides limited protection in fire situations ONLY; it is not effective in spill situations.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

Evacuation: ... Fire: If tank, rail car or tank truck is involved in a fire, isolate for 800 meters (1/2 mile) in all directions; also, consider initial evacuation for 800 meters (1/2 mile) in all directions.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

Fire: CAUTION: All these products have a very low flash point. Use of water spray when fighting fire may be inefficient. Small fires: Dry chemical, CO2, water spray or alcohol-resistant foam. Large fires: Water spray, fog or alcohol-resistant foam. Move containers from fire area if you can do it without risk. Dike fire control water for later disposal; do not scatter the material. Use water spray or fog; do not use straight streams. Fire involving tanks or car/trailer loads: Fight fire from maximum distance or use unmanned hose holders or monitor nozzles. Cool containers with flooding quantities of water until well after fire is out. Withdraw immediately in case of rising sound from venting safety devices or discoloration of tank. ALWAYS stay away from tanks engulfed in fire. For massive fire use unmanned hose holders or monitor nozzles; if this is impossible, withdraw from area and let fire burn.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

Spill or leak: Fully encapsulating, vapor protective clothing should be worn for spills and leaks with no fire. ELIMINATE all ignition sources (no smoking, flares, sparks or flames in immediate area). All equipment used when handling the product must be grounded. Do not touch or walk through spilled material. Stop leak if you can do it without risk. Prevent entry into waterways, sewers, basements or confined areas. A vapor suppressing foam may be used to reduce vapors. Small spills: Absorb with earth, sand or other non-combustible material and transfer to containers for later disposal. Use clean non-sparking tools to collect absorbed material. Large spills: Dike far ahead of liquid spill for later disposal. Water spray may reduce vapor; but may not prevent ignition in closed spaces.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

First aid: Move victim to fresh air. Call 911 or emergency medical service. Apply artificial respiration if victim is not breathing. Do not use mouth-to-mouth method if victim ingested or inhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device. Administer oxygen if breathing is difficult. Remove and isolate contaminated clothing and shoes. In case of contact with substance, immediately flush skin or eyes with running water for at least 20 minutes. Wash skin with soap and water. Keep victim warm and quiet. Effects of exposure (inhalation, ingestion or skin contact) to substance may be delayed. Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves.
[U.S. Department of Transportation. 2000 Emergency Response Guidebook. RSPA P 5800.8 Edition. Washington, D.C: U.S. Government Printing Office, 2000,p. G-131]**QC REVIEWED**

Odor Threshold:

METHYL ALCOHOL DOES NOT HAVE SUITABLE WARNING ODOR ... PROPERTIES EXCEPT @ HIGH CONCN. ... A LEVEL OF 2,000 PPM ... IS BARELY DETECTABLE BY ODOR.
[Clayton, G. D. and F. E. Clayton (eds.). Patty's Industrial Hygiene and Toxicology: Volume 2A, 2B, 2C: Toxicology. 3rd ed. New York: John Wiley Sons, 1981-1982. 4533]**PEER REVIEWED**

Low threshold= 13.1150 mg/cu m; High threshold= 26840 mg/cu m; Irritating concn= 22875 mg/cu m.
[Ruth JH; Am Ind Hyg Assoc J 47: A-142-51 (1986)]**PEER REVIEWED**

Skin, Eye and Respiratory Irritations:

/Methanol/ is a skin and eye irritant.
[Sax, N.I. Dangerous Properties of Industrial Materials. 6th ed. New York, NY: Van Nostrand Reinhold, 1984. 1765]**PEER REVIEWED**

Fire Potential:

DANGEROUS, WHEN EXPOSED TO HEAT OR FLAME
[Sax, N.I. Dangerous Properties of Industrial Materials. 6th ed. New York, NY: Van Nostrand Reinhold, 1984. 1765]**PEER REVIEWED**

NFPA Hazard Classification:

Health: 1. 1= Materials that, on exposure, would cause irritation, but only minor residual injury, including those requiring the use of an approved air-purifying respirator. These materials are only slightly hazardous to health and only breathing protection is needed.
[Fire Protection Guide to Hazardous Materials. 12 ed. Quincy, MA: National Fire Protection Association, 1997.,p. 325-66]**QC REVIEWED**

Flammability: 3. 3= This degree includes Class IB and IC flammable liquids and materials that can be easily ignited under almost all normal temperature conditions. Water may be ineffective in controlling or extinguishing fires in such materials.
[Fire Protection Guide to Hazardous Materials. 12 ed. Quincy, MA: National Fire Protection Association, 1997.,p. 325-66]**QC REVIEWED**

Reactivity: 0. 0= This degree includes materials that are normally stable, even under fire exposure conditions, and that do not react with water. Normal fire fighting procedures may be used.
[Fire Protection Guide to Hazardous Materials. 12 ed. Quincy, MA: National Fire Protection Association, 1997.,p. 325-66]**QC REVIEWED**

Flammable Limits:

Lower limits is 6.0%; Upper limits is 36%
[Fire Protection Guide to Hazardous Materials. 12 ed. Quincy, MA: National Fire Protection Association, 1997.,p. 325-66]**QC REVIEWED**

Flash Point:

12 DEG C (CLOSED CUP)
[The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983. 853]**PEER REVIEWED**

Autoignition Temperature:

464 DEG F (464 DEG C)
[Fire Protection Guide to Hazardous Materials. 12 ed. Quincy, MA: National Fire Protection Association, 1997.,p. 325-66]**QC REVIEWED**

Explosive Limits & Potential:

LOWER 7.3 VOL%; UPPER 36 VOL%
[International Labour Office. Encyclopedia of Occupational Health and Safety. Vols. I&II. Geneva, Switzerland: International Labour Office, 1983. 1356]**PEER REVIEWED**

MODERATE, WHEN EXPOSED TO FLAME
[Sax, N.I. Dangerous Properties of Industrial Materials. 6th ed. New York, NY: Van Nostrand Reinhold, 1984. 1765]**PEER REVIEWED**

Hazardous Reactivities & Incompatibilities:

CAN REACT VIGOROUSLY WITH OXIDIZING MATERIALS.
[Sax, N.I. Dangerous Properties of Industrial Materials. 6th ed. New York, NY: Van Nostrand Reinhold, 1984. 1765]**PEER REVIEWED**

Strong oxidizers.
[NIOSH. NIOSH Pocket Guide to Chemical Hazards. DHHS (NIOSH) Publication No. 97-140. Washington, D.C. U.S. Government Printing Office, 1997. 200]**QC REVIEWED**

Immediately Dangerous to Life or Health:

6000 ppm
[NIOSH. NIOSH Pocket Guide to Chemical Hazards. DHHS (NIOSH) Publication No. 97-140. Washington, D.C. U.S. Government Printing Office, 1997. 200]**QC REVIEWED**

Protective Equipment & Clothing:

Wear appropriate chemical protective gloves, boots, and goggles.
[Association of American Railroads. Emergency Handling of Hazardous Materials in Surface Transportation. Washington, D.C.: Assoc. of American Railroads,Hazardous Materials Systems (BOE), 1987. 444]**PEER REVIEWED**

There is some data suggesting that the breakthrough times of methanol through natural rubber are approximately an hour or more.
[ACGIH; Guidelines Select of Chem Protect Clothing Volume #1 Field Guide p.61 (1983)]**PEER REVIEWED**

Breakthrough times of methanol through nitrile or Viton are greater than one hour reported by (normally) two or more testers.
[ACGIH; Guidelines Select of Chem Protect Clothing Volume #1 Field Guide p.61 (1983)]**PEER REVIEWED**

Breakthrough times of methanol through polyvinyl alcohol or polyvinyl chloride are less (usually significantly less) than one hour reported by (normally) two or more testers.
[ACGIH; Guidelines Select of Chem Protect Clothing Volume #1 Field Guide p.61 (1983)]**PEER REVIEWED**

Recommendations for respirator selection. Max concn for use: 2000 ppm. Respirator Class(es): Any supplied-air respirator.
[NIOSH. NIOSH Pocket Guide to Chemical Hazards. DHHS (NI